Stepanova H, Samankova P, Leva L, Sinkora J, Faldyna M
Department of Immunology, Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic.
Cell Immunol. 2007 Oct;249(2):73-9. doi: 10.1016/j.cellimm.2007.11.007.
In this study, we have characterised postnatal changes in T lymphocyte subsets, especially gammadelta T lymphocytes, in blood, spleen and lymph nodes. Detection was carried out using two-colour flow cytometry and three-colour immunohistochemistry. During ontogeny, there was a significant increase in the total percentage of gammadelta T cells in the spleen and blood. In the lymph nodes, there were no age-dependent changes in the total percentage of gammadelta T cells, but the percentage of the gammadeltaTCR+CD8+ subpopulation significantly increased. The tissue distribution of gammadeltaTCR+CD8+ and gammadeltaTCR+CD8- cells in the lymph nodes is random and not collocated with a particular area of the organ. Furthermore, postnatal development was characterised by an increasing frequency of CD8+CD3+CD4-gammadeltaTCR-, which was compensated by a decreasing proportion of CD4+ lymphocytes. Double positive CD4+CD8+ lymphocytes were rare during the first month of life and a significant age-dependent increase of these cells was found in all the compartments monitored.
在本研究中，我们对血液、脾脏和淋巴结中T淋巴细胞亚群，尤其是γδ T淋巴细胞的出生后变化进行了特征描述。检测采用双色流式细胞术和三色免疫组织化学法。在个体发育过程中，脾脏和血液中γδ T细胞的总百分比显著增加。在淋巴结中，γδ T细胞的总百分比没有年龄依赖性变化，但γδTCR+CD8+亚群的百分比显著增加。γδTCR+CD8+和γδTCR+CD8-细胞在淋巴结中的组织分布是随机的，并不与器官的特定区域并置。此外，出生后发育的特征是CD8+CD3+CD4-γδTCR-的频率增加，这由CD4+淋巴细胞比例的下降所补偿。双阳性CD4+CD8+淋巴细胞在生命的第一个月很少见，并且在所有监测的隔室中都发现这些细胞有显著的年龄依赖性增加。
