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J Bacteriol. 2001 Oct;183(19):5580-8. doi: 10.1128/JB.183.19.5580-5588.2001.
2
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Temporal changes in cold-inducible and uncharacterized Csps under heat and oxidative stress signify a role in bacterial stress response and adaptation.在热应激和氧化应激下，冷诱导型和未表征的Csps的时间变化表明其在细菌应激反应和适应中发挥作用。
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本文引用的文献

1
Escherichia coli CspA-family RNA chaperones are transcription antiterminators.大肠杆菌CspA家族RNA伴侣是转录抗终止因子。
Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):7784-9. doi: 10.1073/pnas.97.14.7784.
2
Adaptive response to cold temperatures and characterization of cspA in Salmonella typhimurium LT2.鼠伤寒沙门氏菌LT2对低温的适应性反应及cspA的特性分析
Antonie Van Leeuwenhoek. 2000 Jan;77(1):13-20. doi: 10.1023/a:1002055719798.
3
A sequence downstream of the initiation codon is essential for cold shock induction of cspB of Escherichia coli.起始密码子下游的序列对于大肠杆菌cspB的冷休克诱导至关重要。
J Bacteriol. 1999 Sep;181(18):5852-4. doi: 10.1128/JB.181.18.5852-5854.1999.
4
Enhancement of translation by the downstream box does not involve base pairing of mRNA with the penultimate stem sequence of 16S rRNA.下游框对翻译的增强作用并不涉及mRNA与16S rRNA倒数第二个茎序列的碱基配对。
Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):8973-8. doi: 10.1073/pnas.96.16.8973.
5
Translational enhancement by an element downstream of the initiation codon in Escherichia coli.大肠杆菌中起始密码子下游元件对翻译的增强作用。
J Biol Chem. 1999 Apr 9;274(15):10079-85. doi: 10.1074/jbc.274.15.10079.
6
Massive presence of the Escherichia coli 'major cold-shock protein' CspA under non-stress conditions.在非应激条件下大肠杆菌“主要冷休克蛋白”CspA大量存在。
EMBO J. 1999 Mar 15;18(6):1653-9. doi: 10.1093/emboj/18.6.1653.
7
CspA, CspB, and CspG, major cold shock proteins of Escherichia coli, are induced at low temperature under conditions that completely block protein synthesis.大肠杆菌的主要冷休克蛋白CspA、CspB和CspG在完全阻断蛋白质合成的条件下于低温时被诱导产生。
J Bacteriol. 1999 Mar;181(6):1827-30. doi: 10.1128/JB.181.6.1827-1830.1999.
8
CspI, the ninth member of the CspA family of Escherichia coli, is induced upon cold shock.CspI是大肠杆菌CspA家族的第九个成员，在冷休克时被诱导产生。
J Bacteriol. 1999 Mar;181(5):1603-9. doi: 10.1128/JB.181.5.1603-1609.1999.
9
Functional determinants of the Escherichia coli fis promoter: roles of -35, -10, and transcription initiation regions in the response to stringent control and growth phase-dependent regulation.大肠杆菌fis启动子的功能决定因素：-35、-10和转录起始区域在严格控制和生长阶段依赖性调控反应中的作用。
J Bacteriol. 1999 Feb;181(4):1269-80. doi: 10.1128/JB.181.4.1269-1280.1999.
10
A superfamily of proteins that contain the cold-shock domain.包含冷休克结构域的蛋白质超家族。
Trends Biochem Sci. 1998 Aug;23(8):286-90. doi: 10.1016/s0968-0004(98)01255-9.

编码鼠伤寒沙门氏菌UK-1中冷休克蛋白的cspH的表达。

Expression of cspH, encoding the cold shock protein in Salmonella enterica serovar Typhimurium UK-1.

作者信息

Kim B H, Bang I S, Lee S Y, Hong S K, Bang S H, Lee I S, Park Y K

机构信息

Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea.

出版信息

J Bacteriol. 2001 Oct;183(19):5580-8. doi: 10.1128/JB.183.19.5580-5588.2001.

DOI:10.1128/JB.183.19.5580-5588.2001
PMID:11544220
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC95449/
Abstract

Both Salmonella enterica serovar Typhimurium and Escherichia coli contain the cspH gene encoding CspH, one of the cold shock proteins (CSPs). In this study, we investigated the expression of cspH in S. enterica serovar Typhimurium and found that it was induced in response to a temperature downshift during exponential phase. The cspH promoter was activated at 37 degrees C, and its mRNA was more stable than the other csp mRNAs at 37 degrees C. Moreover, lacZ expression of the translational cspH-lacZ fusion was induced at that temperature. Interestingly, the cspH mRNA had a much shorter 5'-untranslated region than those in the other cold-shock-inducible genes, and the promoter sequence, which was only 55 bp, was sufficient for cspH expression. The 14-base downstream box located 12 bases downstream of the initiation codon of cspH mRNA was essential for its cold shock activation.

摘要

肠炎沙门氏菌鼠伤寒血清型和大肠杆菌都含有编码冷休克蛋白（CSPs）之一CspH的cspH基因。在本研究中，我们调查了肠炎沙门氏菌鼠伤寒血清型中cspH的表达情况，发现它在指数生长期温度下降时被诱导表达。cspH启动子在37℃时被激活，其mRNA在37℃时比其他csp mRNA更稳定。此外，在该温度下，翻译型cspH-lacZ融合蛋白的lacZ表达被诱导。有趣的是，cspH mRNA的5'-非翻译区比其他冷休克诱导基因的要短得多，仅55 bp的启动子序列就足以实现cspH的表达。位于cspH mRNA起始密码子下游12个碱基处的14碱基下游框对其冷休克激活至关重要。