Durvasula R V, Gumbs A, Panackal A, Kruglov O, Taneja J, Kang A S, Cordon-Rosales C, Richards F F, Whitham R G, Beard C B
Yale University School of Medicine, New Haven, CT, USA.
Med Vet Entomol. 1999 May;13(2):115-9. doi: 10.1046/j.1365-2915.1999.00175.x.
Expression within insects of foreign antiparasitic gene products via microbial symbionts could be used to prevent transmission of vector-borne pathogens to vertebrate hosts. Genetically transformed symbiotic bacteria Rhodococcus rhodnii expressed functional antibody fragments (rDB3 encoding murine V(H)/K which binds progesterone) that were exported into the gut lumen of the triatomine bug Rhodnius prolixus (Hemiptera: Reduviidae), a vector of Chagas disease. Transgenic symbionts were maintained in successive nymphal instars and adults of Rhodnius prolixus despite competition with native untransformed Rhodococcus rhodnii. This is the first description of a functional mammalian antibody fragment expressed in an insect. Our system is a model for constructing paratransgenic insects (insects carrying transformed symbionts) with compromised ability to transmit pathogens.
通过微生物共生体在昆虫体内表达外源抗寄生虫基因产物，可用于防止病媒传播病原体向脊椎动物宿主的传播。基因转化的共生细菌红球菌在锥蝽（半翅目：猎蝽科）肠道腔中表达并分泌功能性抗体片段（编码与孕酮结合的鼠源V(H)/K的rDB3），锥蝽是恰加斯病的传播媒介。尽管与未转化的天然红球菌存在竞争，但转基因共生体仍在锥蝽的若虫龄期和成虫中得以维持。这是首次在昆虫体内表达功能性哺乳动物抗体片段的描述。我们的系统是构建传播病原体能力受损的昆虫（携带转化共生体的昆虫）的模型。
