Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka.
Biotechnology Unit, Industrial Technology Institute, Colombo 07, Sri Lanka.
Biomed Res Int. 2020 Aug 19;2020:5458063. doi: 10.1155/2020/5458063. eCollection 2020.
is the main suspected vector for leishmaniasis in Sri Lanka. Investigations on the presence of aerobic bacteria in the gut of sand flies which evidence a potential approach to control leishmaniasis transmission through a paratransgenic strategy are still not available for the local sand fly populations. Field-caught unfed female sand flies collected from three selected Medical Officer of Health (MOH) areas (Polpithigama, Maho, and Galgamuwa) in Kurunegala District, Sri Lanka from August to December 2018 were used. Prokaryotic 16S ribosomal RNA partial gene was amplified and sequenced. Morphological identification revealed the presence of only one sand fly species, ( = 1,969). A total of 20 organisms belonging to two phyla (Proteobactericea and Furmicutes) were detected within the gut microbial community of the studied sand fly specimens. This study documents the first-ever observation of sp. in the midgut of . The presence of , which is considered as a nonpathogenic bacterium with potential use for paratransgenic manipulation of suggest that it may be used as a delivery vehicle to block the vectorial transmission of parasites. In addition, may be used as a potential candidate to block the parasite development in sand fly vectors since it has evidenced antileishmanial activities in previous investigations. Hence, further studies are required to gain full insight into the potential use of this bacterium in the control of parasites through paratransgenesis.
是斯里兰卡利什曼病的主要可疑媒介。对肠道中需氧细菌的存在进行调查，这些细菌为通过转基因策略控制利什曼病传播提供了一种潜在方法，但对于当地的沙蝇种群，这方面的研究仍尚未开展。2018 年 8 月至 12 月，从斯里兰卡库鲁内格勒区三个选定的卫生官员地区（波皮蒂加马、马霍和加利古瓦）采集未进食的野外捕获雌性沙蝇进行研究。扩增和测序了原核 16S 核糖体 RNA 部分基因。形态鉴定显示仅存在一种沙蝇物种，即（ = 1,969）。在所研究的沙蝇标本的肠道微生物群落中，共检测到 20 种属于两个门（变形菌门和厚壁菌门）的生物。本研究首次观察到 sp. 在 的中肠中存在。存在被认为是一种非致病性细菌，具有用于转基因操作的潜在用途，表明它可能被用作阻断寄生虫载体传播的载体。此外，由于它在先前的研究中表现出抗利什曼原虫活性，因此可能被用作阻断沙蝇媒介中寄生虫发育的潜在候选物。因此，需要进一步的研究来充分了解该细菌在通过转基因控制 寄生虫方面的潜在用途。
