Halm D R, Halm S T
Department of Physiology and Biophysics, Wright State University, Dayton, Ohio 45435, USA.
Am J Physiol. 1999 Sep;277(3):C501-22. doi: 10.1152/ajpcell.1999.277.3.C501.
Crypts of Lieberkühn were isolated from human colon, and differential interference contrast microscopy distinguished goblet and columnar cells. Activation with carbachol (CCh, 100 microM) or histamine (10 microM) released contents from goblet granules. Stimulation with prostaglandin E2 (PGE2, 5 microM) or adenosine (10 microM) did not release goblet granules but caused the apical margin of columnar cells to recede. Goblet volume was lost during stimulation with CCh or histamine (approximately 160 fl/cell), but not with PGE2 or adenosine. Three-quarters of goblet cells were responsive to CCh but released only 30% of goblet volume. Half-time for goblet volume release was 3.7 min. PGE2 stimulated a prolonged fluid secretion that attained a rate of approximately 350 pl/min. Columnar cells lost approximately 50% of apical volume during maximal PGE2 stimulation, with a half-time of 3.3 min. In crypts from individuals with ulcerative colitis, goblet cells were hypersensitive to CCh for release of goblet volume. These results support separate regulation for mucus secretions from goblet cells and from columnar cells, with control mechanisms restricting total release of mucus stores.
从人结肠中分离出利伯kühn隐窝，微分干涉相差显微镜可区分杯状细胞和柱状细胞。用卡巴胆碱（CCh，100微摩尔）或组胺（10微摩尔）激活可使杯状细胞颗粒释放内容物。用前列腺素E2（PGE2，5微摩尔）或腺苷（10微摩尔）刺激不会释放杯状细胞颗粒，但会导致柱状细胞的顶端边缘后退。在用CCh或组胺刺激期间杯状细胞体积减小（约160飞升/细胞），但用PGE2或腺苷刺激时则不会。四分之三的杯状细胞对CCh有反应，但仅释放30%的杯状细胞体积。杯状细胞体积释放的半衰期为3.7分钟。PGE2刺激产生持续的液体分泌，速率约为350皮升/分钟。在最大PGE2刺激期间，柱状细胞顶端体积减少约50%，半衰期为3.3分钟。在溃疡性结肠炎患者的隐窝中，杯状细胞对CCh释放杯状细胞体积高度敏感。这些结果支持杯状细胞和柱状细胞黏液分泌的独立调节，且控制机制限制黏液储存的总释放。
