Levin D B, Huang J
Department of Biology, University of Victoria, Victoria, British Columbia, V8W 3N5, Canada.
J Invertebr Pathol. 1999 Sep;74(2):184-92. doi: 10.1006/jipa.1999.4874.
We determined that the type B nucleopolyhedrovirus of the Egyptian cottonworm, Spodoptera littoralis (SpliNPV), can infect a cell line derived from a grasshopper. We compared the infectivity of SpliNPV in two lepidopteran cell lines (Sf9 and Md210) and in a cell line (MSE4) derived from the western migratory grasshopper, Melanoplus sanguinipes (Orthoptera: Acrididae). Both Sf9 and MSE4 cells were permissive for SpliNPV replication and supported production of viable progeny. Md210 cells were nonpermissive for SpliNPV, and although the virus entered into these cells, they supported neither viral replication nor production of viable progeny. Infection of MSE4 cells with SpliNPV resulted in cytopathic effects within 48 h post infection and complete destruction of the cells within 5 days. Both virions and polyhedra were detected within virus-infected MSE4 cells by transmission electron microscopy. Extracellular virions were detected in the culture medium and were infectious to Sf9 cells, indicating that the MSE4 cells supported production of viable virus progeny.
我们确定，埃及棉铃虫的B型核多角体病毒，即滨海夜蛾核多角体病毒（SpliNPV），能够感染一种源自蚱蜢的细胞系。我们比较了SpliNPV在两种鳞翅目细胞系（Sf9和Md210）以及一种源自西方迁飞蚱蜢，即红胫戟纹蝗（直翅目：蝗科）的细胞系（MSE4）中的感染性。Sf9和MSE4细胞均允许SpliNPV复制，并支持产生有活力的子代病毒。Md210细胞对SpliNPV不敏感，尽管病毒能进入这些细胞，但它们既不支持病毒复制，也不支持产生有活力的子代病毒。用SpliNPV感染MSE4细胞在感染后48小时内导致细胞病变效应，并在5天内使细胞完全破坏。通过透射电子显微镜在病毒感染的MSE4细胞内检测到了病毒粒子和多角体。在培养基中检测到了细胞外病毒粒子，并且它们对Sf9细胞具有感染性，这表明MSE4细胞支持产生有活力的子代病毒。
