Volin M V, Szekanecz Z, Halloran M M, Woods J M, Magua J, Damergis J A, Haines K G, Crocker P R, Koch A E
Lakeside Division, Northwestern University Medical School, Chicago, Illinois 60611, USA.
Exp Mol Pathol. 1999 Aug;66(3):211-9. doi: 10.1006/exmp.1999.2261.
A hallmark of both adjuvant-induced arthritis (AIA) and rheumatoid arthritis is chronic joint inflammation characterized by ingress of leukocytes into the inflamed synovial tissue. The timing of expression of adhesion molecules, which govern the ingress of leukocytes, is important in the orchestration of an inflammatory response. We examined the expression of vascular cell adhesion molecule-1 (VCAM-1), sialo adhesin, platelet and endothelial cell adhesion molecule-1 (PECAM-1), and leukosialin (CD43) in AIA, starting at adjuvant injection (day 0), through the peak of inflammation (day 18 postadjuvant injection), until day 54. VCAM-1 is constitutively expressed on the lining layer and ECs and its expression levels do not change throughout the progression of AIA. Sialoadhesin synovial tissue lining cell expression is decreased after adjuvant injection. In contrast, PECAM-1 expression is increased on synovial tissue lining cells on day 7 and is elevated through day 54 (peaking on day 54 with six-fold more cells expressing PECAM-1). PECAM-1 expression on endothelial cells peaks on day 7 with three-fold more cells expressing it, while on macrophages expression maximizes on day 25 with six-fold more cells expressing PECAM-1. CD43 expression is increased on synovial tissue lining cells, macrophages, neutrophils, and lymphocytes on days 18 and 25, before going back to basal levels. The increased expression of PECAM-1 and CD43 on leukocytes at the height of inflammation in AIA suggests important roles for these adhesion molecules in potentially binding their EC ligands resulting in leukocyte ingress into the synovial tissue.
佐剂诱导性关节炎（AIA）和类风湿性关节炎的一个共同特征是慢性关节炎症，其特点是白细胞侵入发炎的滑膜组织。控制白细胞侵入的黏附分子的表达时机，在炎症反应的协调中很重要。我们研究了血管细胞黏附分子-1（VCAM-1）、唾液酸黏附素、血小板和内皮细胞黏附分子-1（PECAM-1）以及白细胞唾液酸蛋白（CD43）在AIA中的表达情况，从注射佐剂（第0天）开始，经过炎症高峰期（佐剂注射后第18天），直至第54天。VCAM-1在衬里层和内皮细胞上组成性表达，其表达水平在AIA的整个进展过程中没有变化。佐剂注射后，唾液酸黏附素在滑膜组织衬里细胞中的表达减少。相比之下，PECAM-1在第7天在滑膜组织衬里细胞上的表达增加，并在第54天一直升高（在第54天达到峰值，表达PECAM-1的细胞数量增加了六倍）。内皮细胞上PECAM-1的表达在第7天达到峰值，表达它的细胞数量增加了三倍，而在巨噬细胞上，表达在第25天达到最大值，表达PECAM-1的细胞数量增加了六倍。CD43在第18天和第25天在滑膜组织衬里细胞、巨噬细胞、中性粒细胞和淋巴细胞上的表达增加，之后又回到基础水平。在AIA炎症高峰期，白细胞上PECAM-1和CD43表达的增加表明这些黏附分子在潜在地结合其内皮细胞配体从而导致白细胞侵入滑膜组织中发挥重要作用。
