Adrover E, Maestro M L, Sanz-Casla M T, del Barco V, Cerdán J, Fernández C, Balibrea J L
Department of Oncology, Hospital Virgen de la Luz, Cuenca, Spain.
Br J Cancer. 1999 Sep;81(1):122-6. doi: 10.1038/sj.bjc.6690660.
The expression of p53 protein was examined in a series of 111 colorectal cancer adenocarcinomas with a long follow-up. A quantitative luminometric immunoassay (LIA) was used for the measurement of wild-type and mutant p53 protein in extracts from colorectal tumour cytosols, p53 being detected in 42% of the samples (range 0.0-52 ng (mg-1)). Using an arbitrary cut-off value of 2.7 ng mg(-1), 25% of the tumours were classified as manifesting high p53 levels. There was no association of p53 expression with patient age, sex, serum preoperative carcinoembryonic antigen (CEA) levels, tumour site and size, nodal status or TNM stage. Significant and independent correlation was found to exist between high p53 levels and prolonged disease-free survival (P = 0.05) at a median follow-up of 60 months. This survival advantage was most apparent among stage III cancer patients. The results from this study would suggest that expression of high p53 levels appear to be useful in selecting a group of colorectal cancer patients with a better prognosis.
在一系列111例随访时间较长的结肠直肠癌腺癌中检测了p53蛋白的表达。采用定量发光免疫分析法（LIA）测定结肠直肠肿瘤细胞溶质提取物中的野生型和突变型p53蛋白，42%的样本中检测到p53（范围为0.0 - 52 ng（mg-1））。使用2.7 ng mg(-1)的任意临界值，25%的肿瘤被分类为p53水平高。p53表达与患者年龄、性别、术前血清癌胚抗原（CEA）水平、肿瘤部位和大小、淋巴结状态或TNM分期均无关联。在中位随访60个月时，发现p53水平高与无病生存期延长之间存在显著且独立的相关性（P = 0.05）。这种生存优势在III期癌症患者中最为明显。本研究结果表明，高p53水平的表达似乎有助于选择一组预后较好的结肠直肠癌患者。
