Yamaguchi A, Nakagawara G, Kurosaka Y, Nishimura G, Yonemura Y, Miyazaki I
First Department of Surgery, Fukui Medical School, Japan.
Br J Cancer. 1993 Aug;68(2):399-402. doi: 10.1038/bjc.1993.348.
The expression of p53 protein was immunohistochemically studied in formalin-fixed paraffin-embedded biopsy specimens of 203 colorectal carcinomas by use of a monoclonal antibody specific for the p53 protein. PAb1801. p53 protein expression with its reactivity localised in nuclei was found in 121 (59.6%) of the cancers. There was no correlation of p53 immunoreactivity with histological classification, wall invasion, lymphatic invasion, venous invasion, lymph node metastases, or peritoneal metastases. p53-positive cancers were more frequently associated with liver metastasis than p53-negative ones. Patients with p53-positive tumours had significantly poorer prognoses than those with p53-negative tumours. The 5 year survival rate was 58.1% for patients with p53-positive tumours, and 76.3% for those with p53-negative tumours. In Dukes' stage C tumours, an especially good correlation was found between p53 immunoreactivity and prognosis. In addition, patients with p53-positive tumours had higher recurrence rates. The results indicate that p53 immunoreactivity may be a useful prognostic marker of colorectal cancers.
采用对p53蛋白具有特异性的单克隆抗体PAb1801，对203例福尔马林固定、石蜡包埋的结直肠癌活检标本进行免疫组织化学研究，以检测p53蛋白的表达情况。在121例（59.6%）癌症中发现了p53蛋白表达，其反应性定位于细胞核。p53免疫反应性与组织学分类、壁层浸润、淋巴浸润、静脉浸润、淋巴结转移或腹膜转移均无相关性。p53阳性的癌症比p53阴性的癌症更常伴有肝转移。p53阳性肿瘤患者的预后明显比p53阴性肿瘤患者差。p53阳性肿瘤患者的5年生存率为58.1%，p53阴性肿瘤患者为76.3%。在杜克C期肿瘤中，发现p53免疫反应性与预后之间具有特别良好的相关性。此外，p53阳性肿瘤患者的复发率更高。结果表明，p53免疫反应性可能是结直肠癌有用的预后标志物。
