伊尔斯病：玻璃体中膜成分（主要是脂质）的氧化和过氧化产物增加，抗氧化酶和还原型谷胱甘肽减少。

Eales' disease: increased oxidation and peroxidation products of membrane constituents chiefly lipids and decreased antioxidant enzymes and reduced glutathione in vitreous.

作者信息

Sulochana K N, Biswas J, Ramakrishnan S

机构信息

Sankara Nethralaya, Vision Research Foundation Biochemistry Research Department Chennai, India.

出版信息

Curr Eye Res. 1999 Sep;19(3):254-9. doi: 10.1076/ceyr.19.3.254.5312.

DOI:10.1076/ceyr.19.3.254.5312

PMID:10487964

Abstract

PURPOSE

To measure the levels of oxidation and peroxidation products of membrane constituents chiefly lipids produced by oxygen and lipid free radicals as thiobarbituric acid reacting substances (TBARS), reduced glutathione (GSH) and the antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GP) in vitreous samples from patients with Eales' disease and estimate GSH, SOD, GP in the erythrocytes of patients with Eales' disease with active perivasculitis and healed perivasculitis.

METHODS

Vitreous samples on vitrectomy from 11 patients with Eales' disease and 11 patients with diabetic vitreous hemorrhage were used for comparison. For study on erythrocytes, 30 patients of Eales' disease at the active vasculitis stage (group-I), 33 of healed vasculitis stage (group-II) and 25 controls (group-III) were used. The male female ratio was kept the same among the patients and the controls. Oxidation and peroxidation products of membrane constituents chiefly lipids were estimated as TBARS, SOD by its activity to inhibit auto-oxidation of epinephrine, GP by estimating the reduction in the level of GSH and GSH by its colour reaction with 5,5'-dithio bis (2-nitro) benzoic acid (DTNB).

RESULTS

TBARS were increased 6 fold in the vitreous of Eales' patients compared to the samples from those with diabetic vitreous hemorrhage while there was a reduction of 95.9% of SOD and 84.2% of GSH. Activity of SOD, GP and the levels of GSH in the erythrocytes were reduced (%) by 81.6, 65 and 56.5 respectively in group I and 22,46.4 and 29.2% respectively in group II. The values are statistically significant.

CONCLUSIONS

Increased levels of TBARS and decreased levels of SOD and GSH in the vitreous could explain inflammation, retinal damage and neovascularization in patients with Eales' disease. Decrease of SOD, GP and GSH is found in erythrocytes both in the active perivasculitis stage and the healed perivasculitis stage. Treatment of Eales' disease with antioxidants vitamin E, C as also vitamin A may have a beneficial effect.

摘要

目的

测定伊尔斯病患者玻璃体液中主要由氧自由基和脂质自由基产生的膜成分（主要是脂质）的氧化和过氧化产物，即硫代巴比妥酸反应物质（TBARS）、还原型谷胱甘肽（GSH）以及抗氧化酶超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GP）的水平，并评估处于活动期血管周围炎和愈合期血管周围炎的伊尔斯病患者红细胞中的GSH、SOD、GP水平。

方法

使用11例伊尔斯病患者和11例糖尿病性玻璃体积血患者玻璃体切除术中获取的玻璃体液样本进行比较。对于红细胞研究，选取30例处于活动期血管炎阶段的伊尔斯病患者（I组）、33例处于愈合期血管炎阶段的患者（II组）以及25例对照者（III组）。患者和对照者的男女比例保持相同。通过TBARS评估主要脂质的膜成分的氧化和过氧化产物，通过SOD抑制肾上腺素自氧化的活性来测定SOD，通过评估GSH水平的降低来测定GP，通过GSH与5,5'-二硫代双（2-硝基）苯甲酸（DTNB）的显色反应来测定GSH。