伊尔斯病：活性氧中间体和脂质过氧化物积累，抗氧化剂减少，导致炎症、新生血管形成和视网膜损伤。

Eales' disease: accumulation of reactive oxygen intermediates and lipid peroxides and decrease of antioxidants causing inflammation, neovascularization and retinal damage.

作者信息

Bhooma V, Sulochana K N, Biswas J, Ramakrishnan S

机构信息

Biochemistry Research Department, Vision Research Foundation, Madras, India.

出版信息

Curr Eye Res. 1997 Feb;16(2):91-5. doi: 10.1076/ceyr.16.2.91.5096.

DOI:10.1076/ceyr.16.2.91.5096

PMID:9068938

Abstract

PURPOSE

To measure the blood levels of oxygen and lipid-free radicals as lipid peroxidation products and of vitamins E, C and A, in order to explain intraocular inflammation, retinal neovascularization and detachment in Eales' disease.

METHODS

Levels of the lipid peroxidation products produced by oxygen and lipid free radicals (estimated as thiobarbituric acid reactive substances-TBARS), vitamin E with ferric chloride, vitamin C with 2,6-dichlorophenol-indophenol and vitamin A with antimony trichloride were all evaluated in three groups of subjects between 20 and 40 years of age. Twenty three were patients with Eales' disease in the active perivasculitis stage (Group I), 19 were patients with Eales' disease in the healed-perivasculitis stage (Group II), and 46 were healthy volunteers (Group III), used as normal controls. Patients in each group were subdivided into two age groups, 20-30 and 31-40 years, each group containing both sexes.

RESULTS

The results showed a 4.6- and 5.6-fold increase in the levels of TBARS in erythrocytes of patients with Eales' disease in the active perivasculitis stage (Group I) and a 2-fold increase in patients with Eales' disease in the healed perivasculitis stage (Group II), when compared to levels in the controls (Group III). On the other hand, there was a decrease of 75 and 76.2% in the levels of vitamin E in serum, 34 and 40.9% of vitamin C in plasma and 72.8 and 67% of vitamin A in serum in patients with Eales' disease in the active perivasculitis stage (Group I), as compared to the controls (Group III). Also decreases of 56 and 43.5% of vitamin E in serum, 26.8 and 12.5% of vitamin C in plasma and 50.5 and 49.4% of vitamin A in serum were found in patients with Eales' disease in the healed perivasculitis stage (Group II) as compared to the healthy controls (Group III). The two values of variations between the patients and the normal control group given above for TBARS, vitamins E,C and A are for the two age groups, 20-30 and 31-40 respectively. The differences were statistically significant (P < 0.01 to 0.001).

CONCLUSIONS

Lowered levels of antioxidant vitamins E and C and consequent accumulation of oxygen and lipid free radicals, or vice versa, could explain the inflammation, neovascularization and retinal pathology in patients with Eales' disease. Also, vitamin A deficiency could aggravate retinal illness.

摘要

目的

测量血液中氧和脂质自由基（作为脂质过氧化产物）以及维生素E、C和A的水平，以解释伊尔斯病中的眼内炎症、视网膜新生血管形成和视网膜脱离。

方法

对三组年龄在20至40岁之间的受试者评估了氧和脂质自由基产生的脂质过氧化产物水平（以硫代巴比妥酸反应性物质-TBARS估算）、用氯化铁测定的维生素E水平、用2,6-二氯酚靛酚测定的维生素C水平以及用三氯化锑测定的维生素A水平。23例处于活动期血管周炎阶段的伊尔斯病患者为第一组，19例处于愈合期血管周炎阶段的伊尔斯病患者为第二组，46例健康志愿者为第三组作为正常对照。每组患者又分为两个年龄组，20-30岁和31-40岁，每组均包含男女两性。

结果

结果显示，与对照组（第三组）相比，处于活动期血管周炎阶段的伊尔斯病患者（第一组）红细胞中TBARS水平增加了4.6倍和5.6倍，处于愈合期血管周炎阶段的伊尔斯病患者（第二组）增加了2倍。另一方面，与对照组（第三组）相比，处于活动期血管周炎阶段的伊尔斯病患者（第一组）血清中维生素E水平降低了75%和76.2%，血浆中维生素C水平降低了34%和40.9%，血清中维生素A水平降低了72.8%和67%。与健康对照组（第三组）相比，处于愈合期血管周炎阶段的伊尔斯病患者（第二组）血清中维生素E水平也分别降低了56%和43.5%，血浆中维生素C水平降低了26.8%和12.5%，血清中维生素A水平降低了50.5%和49.4%。上述患者与正常对照组之间TBARS、维生素E、C和A的两组变化值分别针对20-30岁和31-40岁这两个年龄组。差异具有统计学意义（P<0.01至0.001）。