伊尔斯病患者中8-羟基脱氧鸟苷的积累及其与抗氧化参数的关系：对抗氧化治疗的启示

Accumulation of 8-hydroxydeoxyguanosine and its relationship with antioxidant parameters in patients with Eales' disease: implications for antioxidant therapy.

作者信息

Rajesh Mohanraj, Ramesh Atmakuru, Ravi Perumal Elumalai, Balakrishnamurthy Prakhya, Coral Karunakaran, Punitham Ranganathan, Sulochana Konerirajapuram N, Biswas Joytirmay, Ramakrishnan Sivaramakrishnan

机构信息

Biochemistry Research Department, Vision Research Foundation, Sankara Nethralaya, Chennai, India.

出版信息

Curr Eye Res. 2003 Aug;27(2):103-10. doi: 10.1076/ceyr.27.2.103.15951.

DOI:10.1076/ceyr.27.2.103.15951

PMID:14632162

Abstract

PURPOSE

Eales' disease (ED) is an idiopathic retinal inflammatory disease, which affects the retina of young adult males. Oxidative stress has been associated with the development of various inflammatory diseases. Accumulation of DNA oxidation products has been considered as an early biomarker for assessing oxidative tissue damage. To our knowledge there are no human clinical reports available on the accumulation of DNA adducts in ocular inflammatory diseases. Hence the aim of the present study was to determine the levels of 8-hydroxy-2'deoxyguanosine (8-OHdG) and to correlate it with the antioxidant and oxidant parameters in patients with ED and healthy control subjects.

METHODS

Twenty-two patients with ED [12 in active perivasculitis (AV) and 10 in healed vasculitis stages (HV)] were recruited for the study. 14 healthy volunteers were included as control. 8-OHdG was determined in their leukocytes by Gas chromatography-Mass Spectrometry (GC/MS) technique. Glutathione levels were determined in plasma, while Superoxide dismutase (SOD) activity and thiobarbituricacid reactive substances (TBARS) content were determined in their erythrocytes.

RESULTS

Our results indicated that 8-OHdG levels were elevated by 3 and 1.9 folds respectively in AV and HV stages of patients with ED, when compared with healthy control subjects. Increased 8-OHdG levels correlated with diminished SOD activity and decreased GSH content in patients with ED.

CONCLUSIONS

To our knowledge, this is the first report of DNA adduct accumulation in patients with ED. Increased DNA adduct accumulation correlated with the severity of the disease, and they lie in parallel with diminished antioxidant capacity observed in patients with ED. Based on the observations from our present work and our earlier studies, we reiterate that oxidative stress is involved in the disease process. Hence we believe antioxidant vitamin E and C supplementation might be beneficial to patients with ED.

摘要

目的

伊尔斯病（ED）是一种特发性视网膜炎症性疾病，影响年轻成年男性的视网膜。氧化应激与多种炎症性疾病的发生有关。DNA氧化产物的积累被认为是评估氧化组织损伤的早期生物标志物。据我们所知，尚无关于眼部炎症性疾病中DNA加合物积累的人类临床报告。因此，本研究的目的是测定8-羟基-2'-脱氧鸟苷（8-OHdG）水平，并将其与ED患者和健康对照者的抗氧化和氧化参数相关联。

方法

招募了22例ED患者[12例处于活动性血管周围炎（AV）阶段，10例处于血管炎愈合阶段（HV）]进行研究。纳入14名健康志愿者作为对照。通过气相色谱-质谱（GC/MS）技术测定其白细胞中的8-OHdG。测定血浆中的谷胱甘肽水平，同时测定其红细胞中的超氧化物歧化酶（SOD）活性和硫代巴比妥酸反应性物质（TBARS）含量。