达松维尔诺卡氏菌血液分离株的分子鉴定
Molecular identification of a Nocardiopsis dassonvillei blood isolate.
作者信息
Beau F, Bollet C, Coton T, Garnotel E, Drancourt M
机构信息
Laboratoire de Bactériologie, Assistance Publique-Hôpitaux de Marseille, Hôpital d'Instruction des Armées Alphonse Laveran, Marseille, France.
出版信息
J Clin Microbiol. 1999 Oct;37(10):3366-8. doi: 10.1128/JCM.37.10.3366-3368.1999.
Abstract
Nocardiopsis dassonvillei is an environmental aerobic actinomycete seldom isolated in cutaneous and pulmonary infections. We herein report the first N. dassonvillei blood isolate in a patient hospitalized for cholangitis. Although morphological characteristics and biochemical tests allowed a presumptive identification of this isolate, cell wall fatty acid chromatographic analysis confirmed identification at the genus level, and 16S rRNA gene sequencing achieved definite identification. This study illustrates the usefulness of 16S rRNA gene sequencing as a routine method for the identification of actinomycetes.
摘要
达松维尔诺卡氏菌是一种环境需氧放线菌，很少在皮肤和肺部感染中分离得到。我们在此报告了第一例从一名因胆管炎住院患者血液中分离出的达松维尔诺卡氏菌。尽管形态学特征和生化试验可对该分离株进行初步鉴定，但细胞壁脂肪酸色谱分析在属水平上确认了鉴定结果，而16S rRNA基因测序实现了确切鉴定。本研究说明了16S rRNA基因测序作为放线菌鉴定常规方法的实用性。
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