Hozzein Wael N, Goodfellow Michael
Botany Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
Int J Syst Evol Microbiol. 2008 Nov;58(Pt 11):2520-4. doi: 10.1099/ijs.0.65014-0.
The taxonomic status of an unknown actinomycete isolated from a sand-dune soil was established using a polyphasic approach. Isolate S186(T) had chemotaxonomic and morphological properties consistent with its classification in the genus Nocardiopsis, grew on agar plates at NaCl concentrations of up to 15 % (w/v) and formed a distinct phyletic line in the Nocardiopsis 16S rRNA gene sequence tree. Its closest phylogenetic neighbours were Nocardiopsis chromatogenes, Nocardiopsis composta, Nocardiopsis gilva and Nocardiopsis trehalosi, with sequence similarity to the various type strains of 96.9 %, but it was readily distinguished from the type strains of these and related species using a range of phenotypic properties. It is apparent from the genotypic and phenotypic data that strain S186(T) belongs to a novel species of the genus Nocardiopsis, for which the name Nocardiopsis arabia sp. nov. is proposed. The type strain is S186(T) (=CGMCC 4.2057(T) =DSM 45083(T)).
采用多相分类方法确定了从沙丘土壤中分离出的一种未知放线菌的分类地位。菌株S186(T)具有化学分类学和形态学特性，与其在诺卡氏菌属中的分类一致，能在NaCl浓度高达15%（w/v）的琼脂平板上生长，并在诺卡氏菌16S rRNA基因序列树中形成一个独特的进化分支。其最接近的系统发育邻居是产色诺卡氏菌、复合诺卡氏菌、金色诺卡氏菌和海藻糖诺卡氏菌，与各种模式菌株的序列相似性为96.9%，但利用一系列表型特征可将其与这些及相关物种的模式菌株轻松区分开来。从基因型和表型数据来看，菌株S186(T)属于诺卡氏菌属的一个新物种，为此提出新名称阿拉伯诺卡氏菌（Nocardiopsis arabia sp. nov.）。模式菌株为S186(T)（=CGMCC 4.2057(T)=DSM 45083(T)）。
