Hwang J S, Lee J S, Goo T W, Kang H A, Sohn H R, Kim H R, Kwon O Y
National Sericulture and Entomology Research, R. D. A., Suwon, Korea.
Z Naturforsch C J Biosci. 1999 Jul-Aug;54(7-8):587-94. doi: 10.1515/znc-1999-7-821.
The phylogenetic relationships between Bombyx mori and Bombyx mandarina species of Bombycidae, and Antheraea yamamai and Antheraea pernyi species of Saturniidae were investigated based on mtDNA RFLP and cytochrome oxidase I gene. The sizes of the mtDNA of all the species were estimated at approximately 16 kbp +/- 500 bp by total length of all the restricted fragments and no variation in size was recognized. Of the fourteen different restriction endonucleases used, BamHI, HindIII, PstI, EcoRI and XbaI showed RFLP. Among these, only HindIII showed RFLP between B. mori and B. mandarina. A comparative analysis of sequences was also conducted with the mitochondrial cytochrome oxidase I genes of each species. The results indicated that B. mori shared a 97%, 85% and 87% sequence identity with B. mandarina, A. yamamai and A. pernyi, respectively. B. mandarina shared a 87% and 88% sequence identity with A. yamamai and A. pernyi, respectively. A. yamamai shared 92% sequence identity with A. pernyi. The results of the phylogenetic analysis exhibited monophyly and confidence limits of more than 99% in all trees for both Bombycidae and Saturniidae.
基于线粒体DNA限制性片段长度多态性（mtDNA RFLP）和细胞色素氧化酶I基因，对蚕蛾科的家蚕和野桑蚕物种，以及天蚕蛾科的日本柞蚕和柞蚕物种之间的系统发育关系进行了研究。通过所有限制性片段的总长度估计，所有物种的线粒体DNA大小约为16 kbp±500 bp，且未发现大小变异。在所使用的14种不同限制性内切酶中，BamHI、HindIII、PstI、EcoRI和XbaI显示出限制性片段长度多态性。其中，只有HindIII在家蚕和野桑蚕之间显示出限制性片段长度多态性。还对每个物种的线粒体细胞色素氧化酶I基因进行了序列比较分析。结果表明，家蚕与野桑蚕、日本柞蚕和柞蚕的序列同一性分别为97%、85%和87%。野桑蚕与日本柞蚕和柞蚕的序列同一性分别为87%和88%。日本柞蚕与柞蚕的序列同一性为92%。系统发育分析结果显示，蚕蛾科和天蚕蛾科的所有树状图均呈现单系性，置信度超过99%。
