Mahendran B, Ghosh S K, Kundu S C
Department of Biotechnology, Indian Institute of Technology, Kharagpur 721 302, India.
J Genet. 2006 Apr;85(1):31-8. doi: 10.1007/BF02728967.
We have examined the molecular-phylogenetic relationships between nonmulberry and mulberry silkworm species that belong to the families Saturniidae, Bombycidae and Lasiocampidae using 16S ribosomal RNA (16S rRNA) and cytochrome oxidase subunit I (coxI) gene sequences. Aligned nucleotide sequences of 16S rRNA and coxI from 14 silk-producing species were used for construction of phylogenetic trees by maximum likelihood and maximum parsimony methods. The tree topology on the basis of 16S rRNA supports monophyly for members of Saturniidae and Bombycidae. Weighted parsimony analysis weighted towards transversions relative to transitions (ts, tv4) for coxI resulted in more robust bootstrap support over unweighted parsimony and favours the 16S rRNA tree topology. Combined analysis reflected clear biogeographic pattern, and agrees with morphological and cytological data.
我们利用16S核糖体RNA（16S rRNA）和细胞色素氧化酶亚基I（coxI）基因序列，研究了属于天蚕蛾科、蚕蛾科和枯叶蛾科的非桑蚕和桑蚕物种之间的分子系统发育关系。来自14个产丝物种的16S rRNA和coxI的比对核苷酸序列，通过最大似然法和最大简约法用于构建系统发育树。基于16S rRNA的树形拓扑结构支持天蚕蛾科和蚕蛾科成员的单系性。相对于转换（ts，tv4）对颠换加权的加权简约分析，相较于未加权简约分析，coxI产生了更强有力的自展支持，并支持16S rRNA树形拓扑结构。联合分析反映出清晰的生物地理模式，且与形态学和细胞学数据一致。
