Arunkumar K P, Metta Muralidhar, Nagaraju J
Laboratory of Molecular Genetics, Centre for DNA Fingerprinting and Diagnostics, ECIL Road, Nacharam, Hyderabad 500076, India.
Mol Phylogenet Evol. 2006 Aug;40(2):419-27. doi: 10.1016/j.ympev.2006.02.023. Epub 2006 Apr 27.
Molecular phylogeny of some of the economically important silkmoths was derived using three mitochondrial genes, 12S rRNA, 16S rRNA, and COI, and the control region (CR). Maximum likelihood (ML) analyses showed two distinct clades, one consisting of moths from Bombycidae family and the other from Saturniidae family. The mitochondrial CR showed length polymorphisms with indels. The ML analyses for complete mitochondrial genome sequences of Bombyx mori (strains Aojuku, C108, Backokjam, and Xiafang), Japanese and Chinese strains of B. mandarina (Japanese mandarina and Chinese mandarina) and, Antheraea pernyi revealed two distinct clades, one comprising of B. mori strains and the other with B. mandarina, and A. pernyi forming an outgroup. Pairwise distances revealed that all of the strains of B. mori studied are closer to Chinese than to Japanese mandarina. Phylogenetic analyses based on whole mitochondrial genome sequences, the finding of a tandem triplication of a 126bp repeat element only in Japanese mandarina, and chromosome number variation in B. mandarina suggest that B. mori must have shared its recent common ancestor with Chinese mandarina. Another wild species of the Bombycidae family, Theophila religiosa, whose phylogenetic status was not clear, clustered together with the other bombycid moths in the study. Analysis of the interspecific hybrid, A. proylei gave evidence for paternal inheritance of mitochondrial DNA.
利用三个线粒体基因（12S rRNA、16S rRNA和COI）以及控制区（CR），推导了一些具有重要经济价值的蚕蛾的分子系统发育。最大似然（ML）分析显示出两个不同的进化枝，一个由蚕蛾科的蛾类组成，另一个由天蚕蛾科的蛾类组成。线粒体CR显示出具有插入缺失的长度多态性。对家蚕（青熟、C108、Backokjam和夏芳品系）、日本和中国的野桑蚕品系（日本野桑蚕和中国野桑蚕）以及柞蚕的完整线粒体基因组序列进行的ML分析显示出两个不同的进化枝，一个由家蚕品系组成，另一个由野桑蚕组成，柞蚕形成一个外类群。成对距离显示，所研究的所有家蚕品系与中国野桑蚕的亲缘关系比与日本野桑蚕更近。基于全线粒体基因组序列的系统发育分析、仅在日本野桑蚕中发现的126bp重复元件的串联三倍体以及野桑蚕的染色体数目变异表明，家蚕必定与中国野桑蚕有着最近的共同祖先。蚕蛾科的另一个野生种——柳蚕，其系统发育地位尚不明确，在该研究中与其他蚕蛾科蛾类聚集在一起。对种间杂交种——A. proylei的分析为线粒体DNA的父系遗传提供了证据。
