Long L H, Lan A N, Hsuan F T, Halliwell B
Department of Biochemistry, National University of Singapore, Singapore.
Free Radic Res. 1999 Jul;31(1):67-71. doi: 10.1080/10715769900300611.
The ability of several beverages to generate hydrogen peroxide was demonstrated by direct measurement using the ferrous ion oxidation-xylenol orange (FOX) assay. Tea and coffee could generate H2O2 to achieve levels over 100 microM, but cocoa did not. Milk decreased net H2O2 production by beverages and showed some ability to remove H2O2 itself, apparently not because of catalase activity. Hence several of the beverages commonly drunk by humans show a complex mixture of anti- and pro-oxidant abilities.
通过使用亚铁离子氧化-二甲苯酚橙（FOX）测定法直接测量，证明了几种饮料产生过氧化氢的能力。茶和咖啡能够产生过氧化氢，使其水平超过100微摩尔，但可可则不能。牛奶降低了饮料中过氧化氢的净产量，并且显示出自身具有一定的去除过氧化氢的能力，这显然不是由于过氧化氢酶的活性。因此，人类常饮用的几种饮料表现出抗氧化和促氧化能力的复杂混合。
