Ou P, Wolff S P
Department of Medicine, University College London Medical School, UK.
J Biochem Biophys Methods. 1996 Jan 11;31(1-2):59-67. doi: 10.1016/0165-022x(95)00039-t.
We describe a discontinuous method for the measurement of catalase using the Ferrous Oxidation in Xylenol orange (FOX) assay. Samples containing catalase are incubated with H2O2 for varying time intervals prior to rapid mixing of aliquots of the incubation mixtures with FOX reagent, which measures residual H2O2. Absorbance is then read at 560 nm after 30-min incubation at room temperature. Decay of H2O2 is proportional to catalase activity in the original sample. An adaptation of the method involves exposure of intact cells to H2O2-generating systems in the presence of aminotriazole. The extent of catalase inactivation allows estimates of total H2O2 accumulation within the cell.
我们描述了一种使用二甲酚橙亚铁氧化（FOX）测定法测量过氧化氢酶的间断方法。含有过氧化氢酶的样品在与过氧化氢孵育不同时间间隔后，将等分的孵育混合物与FOX试剂快速混合，该试剂用于测量残留的过氧化氢。然后在室温下孵育30分钟后，于560nm处读取吸光度。过氧化氢的衰减与原始样品中的过氧化氢酶活性成正比。该方法的一种改进是在氨基三唑存在下，将完整细胞暴露于过氧化氢生成系统中。过氧化氢酶失活的程度可用于估计细胞内过氧化氢的总积累量。
