Long L H, Evans P J, Halliwell B
Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, 119260, Singapore.
Biochem Biophys Res Commun. 1999 Sep 7;262(3):605-9. doi: 10.1006/bbrc.1999.1263.
The presence of hydrogen peroxide, at levels sometimes exceeding 100 microM, in human urine samples was established by three different assay methods: 2-oxoglutarate decarboxylation and the ferrous oxidation-xylenol orange (FOX) assay and an oxygen electrode. Detected levels of H(2)O(2) were decreased by addition of superoxide dismutase. We conclude that urine contains autooxidizable molecules that, upon exposure to 21% O(2), undergo rapid superoxide-dependent autooxidation reactions to generate H(2)O(2). The exposure of human tissues to hydrogen peroxide may be greater than is commonly supposed, which has implications in relation to the proposed role of this species in cell signaling.
通过三种不同的检测方法证实，人类尿液样本中存在过氧化氢，其含量有时超过100微摩尔：2-氧代戊二酸脱羧反应、亚铁氧化-二甲酚橙（FOX）检测法和氧电极法。添加超氧化物歧化酶后，检测到的过氧化氢水平降低。我们得出结论，尿液中含有可自动氧化的分子，这些分子在暴露于21%氧气时，会发生快速的超氧化物依赖性自动氧化反应以生成过氧化氢。人体组织接触过氧化氢的程度可能比通常认为的要高，这与该物质在细胞信号传导中所起的作用有关。
