人乳头瘤病毒16 E6与食管癌细胞的核基质相关。
Human papillomavirus 16 E6 is associated with the nuclear matrix of esophageal carcinoma cells.
作者信息
Chen H B, Chen L, Zhang J K, Shen Z Y, Su Z J, Cheng S B, Chew E C
机构信息
Department of Histology and Embryology, Shantou University Medical College, Guangdong Province, China.
出版信息
World J Gastroenterol. 2001 Dec;7(6):788-91. doi: 10.3748/wjg.v7.i6.788.
Abstract
AIM
To explore the etiologic role of HPV infection in esophageal carcinoma, and the association of HPV-16 E6 with the nuclear matrix of carcinoma cells.
METHODS
Two esophageal carcinoma cell lines,EC/CUHK1 and EC/CUHK2, were tested for HPV-16 E6 subgenetic fragment by polymerase chain reaction amplification of virus DNA associated nuclear matrix. RT-PCR and immunocytochemistry were also used to visualize the expression of E6 subgene in the cells.
RESULTS
The HPV-16 E6 subgenetic fragment was found to be present in nuclear matrix-associated DNA, E6 oncoprotein localized in the nucleus where it is tightly associated with nuclear matrix after sequential extraction in EC/CUHK2 cells. It was not detected, however, in EC/CUHK1 cells.
CONCLUSION
The interaction between HPV-16 E6 and nuclear matrix may contribute to the virus induced carcinogenesis in esophageal carcinoma.
摘要
目的
探讨人乳头瘤病毒（HPV）感染在食管癌发病中的作用，以及HPV-16 E6与癌细胞核基质的关系。
方法
采用聚合酶链反应扩增与核基质相关的病毒DNA，检测两种食管癌细胞系EC/CUHK1和EC/CUHK2中的HPV-16 E6亚基因片段。同时运用逆转录聚合酶链反应（RT-PCR）和免疫细胞化学方法观察细胞中E6亚基因的表达情况。
结果
在EC/CUHK2细胞中，经连续抽提后发现HPV-16 E6亚基因片段存在于与核基质相关的DNA中，E6癌蛋白定位于细胞核内，并与核基质紧密结合；而在EC/CUHK1细胞中未检测到。
结论
HPV-16 E6与核基质之间的相互作用可能在食管癌病毒诱导的致癌过程中发挥作用。
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