Williams P S, Zborowski M, Chalmers J J
Department of Chemistry and Geochemistry, Colorado School of Mines, Golden 80401, USA.
Anal Chem. 1999 Sep 1;71(17):3799-807. doi: 10.1021/ac990284+.
The quadrupole magnetic cell sorter is a form of split-flow thin-channel (SPLITT) separation device. It employs a quadrupole magnetic field and annular channel geometry. Immunomagnetic labels are used to bind to specific receptors on the surface of the cells of interest. It is the interaction of these labels with the magnetic field that brings about the selective isolation of these cells. The SPLITT separation devices have generally been based on parallel-plate geometry, usually with effectively constant field strength applied across the channel thickness. The nonconstant field strength and annular channel geometry of the magnetic cell sorter require that a new strategy be developed for optimization of inlet and outlet flow rates. We present such a strategy here based on a consideration of certain specific cell trajectories within the system.
四极磁细胞分选仪是分流薄通道（SPLITT）分离装置的一种形式。它采用四极磁场和环形通道结构。免疫磁标记用于与感兴趣细胞表面的特定受体结合。正是这些标记与磁场的相互作用实现了这些细胞的选择性分离。SPLITT分离装置通常基于平行板结构，通常在通道厚度上施加有效恒定的场强。磁细胞分选仪的非恒定场强和环形通道结构要求开发一种新策略来优化进样和出样流速。我们在此基于对系统内某些特定细胞轨迹的考虑提出这样一种策略。
