Lima L H, De Marco J L, Ulhoa C J, Felix C R
Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia, Empresa Brasileira de Pesquisa Agropecuária, Brasil.
Folia Microbiol (Praha). 1999;44(1):45-9. doi: 10.1007/BF02816220.
A Trichoderma sp. isolate, hereafter called T6, produces a 46-kDa endochitinase (CHIT 46) which had been shown to drastically affect in vitro the cell walls of the phytopathogens Sclerotium rolfsii and Rhizoctonia solani. We attempted to gain insight into its properties. The CHIT 46 N-terminal amino acid sequence shares a very high homology with other fungal chitinases. Western blot analysis using polyclonal antibodies anti-CHIT 46 revealed that this enzyme is immunologically distinct from other proteins produced by the same Trichoderma isolate T6, but is immunologically identical with proteins having equivalent molar mass, probably chitinases, produced by other Trichoderma spp. isolates. In addition, the antibodies revealed also that a substantial amount of this enzyme is secreted into the culture medium 2 d after the Trichoderma isolate T6 comes into contact with chitin.
一种木霉属菌株，以下称为T6，可产生一种46 kDa的内切几丁质酶（CHIT 46），该酶已被证明在体外能显著影响植物病原菌罗氏白绢病菌和立枯丝核菌的细胞壁。我们试图深入了解其特性。CHIT 46的N端氨基酸序列与其他真菌几丁质酶具有很高的同源性。使用抗CHIT 46多克隆抗体进行的蛋白质免疫印迹分析表明，这种酶在免疫上与同一木霉属菌株T6产生的其他蛋白质不同，但与其他木霉属菌株产生的具有相同摩尔质量的蛋白质（可能是几丁质酶）在免疫上相同。此外，这些抗体还表明，在木霉属菌株T6与几丁质接触2天后，大量这种酶被分泌到培养基中。
