Sterpetti A V, Cucina A, Randone B, Graziano P, Stipa F, Corvino V, Cavallaro G, Palmieri I, Amato D, Polistena A, Cavallaro A
I Istituto Clinica Chirurgica, University of Rome La Sapienza, Italy.
Eur J Surg. 1999 Aug;165(8):772-6. doi: 10.1080/11024159950189564.
To assess the role of polyclonal antibodies to basic fibroblast growth factor (bFGF) in inhibiting myointimal hyperplasia after insertion of polytetrafluoroethylene (PTFE) grafts in rats.
Experimental study.
University laboratory, Italy.
24 inbred Lewis rats.
A segment of PTFE I cm long was interposed in the abdominal aorta. The animals were randomised in two groups, n = 12 in each. The first were given polyclonal antibodies to bFGF at the time of operation, and for the first two postoperative days; and the second were given non-specific IgG at the same time periods.
Two animals died during the immediate postoperative period of anaesthetic complications. 12 animals (6 in each group) were killed 7 days postoperatively (24 hours after injection of 5-bromo-deoxyuridine BrdU) to assess smooth muscle cell proliferation. The remaining 10 animals (5 in each group) were killed after 1 month to assess the degree of anastomotic myointimal hyperplasia.
Antibodies to bFGF resulted in less smooth muscle cell proliferation at the anastomoses as well as anastomotic myointimal hyperplasia. Smooth muscle cell proliferation was reduced to about half in animals treated with anti-bFGF antibodies. Neointimal thickness was reduced in treated animals.
We conclude that after PTFE arterial grafting there is increased production of bFGF at the anastomotic regions that leads to smooth muscle cell proliferation and formation of myointimal hyperplasia. Agents that reduce the production of bFGF may also reduce the development of myointimal hyperplasia after PTFE arterial grafting.
评估抗碱性成纤维细胞生长因子（bFGF）多克隆抗体在抑制大鼠插入聚四氟乙烯（PTFE）移植物后肌内膜增生中的作用。
实验研究。
意大利大学实验室。
24只近交系Lewis大鼠。
将一段1厘米长的PTFE置于腹主动脉中。动物被随机分为两组，每组12只。第一组在手术时及术后前两天给予抗bFGF多克隆抗体；第二组在相同时间段给予非特异性IgG。
2只动物在术后即刻因麻醉并发症死亡。术后7天（注射5-溴脱氧尿苷BrdU后24小时）处死12只动物（每组6只）以评估平滑肌细胞增殖。其余10只动物（每组5只）在1个月后处死以评估吻合口肌内膜增生程度。
抗bFGF抗体导致吻合口处平滑肌细胞增殖及吻合口肌内膜增生减少。用抗bFGF抗体治疗的动物中平滑肌细胞增殖减少至约一半。治疗动物的新生内膜厚度降低。
我们得出结论，PTFE动脉移植后，吻合区域bFGF产生增加，导致平滑肌细胞增殖和肌内膜增生形成。减少bFGF产生的药物也可能减少PTFE动脉移植后肌内膜增生的发展。
