Sterpetti A V, Cucina A, Lepidi S, Randone B, Stipa F, Aromatario C, Travi D, D'Angelo L S, Cavallaro A, Stipa S
First Department of Surgery, University of Rome, La Sapienza, Italy.
J Vasc Surg. 1996 Apr;23(4):568-75. doi: 10.1016/s0741-5214(96)80034-6.
The factors that lead to myointimal hyperplasia (MH) in arterial vein grafts (AVGs) are unknown. Platelet-derived growth factor (PDGF) and basic fibroblastic growth factor (bFGF) are two powerful mitogens for smooth muscle cells that have been implicated in the genesis of MH. The aim of this study was to analyze the correlation between progression and regression of MH and production of PDGF and bFGF in experimental vein grafts.
In 64 inbred Lewis rats, a 1-cm segment of inferior vena cava was inserted at the level of the abdominal aorta. The segments of inferior vena cava were obtained from syngenic rats. In 48 rats, the AVG was explanted 3 days (n = 8), 7 days (n = 8), 4 weeks (n = 24), and 12 weeks (n = 8) after surgery. In 16 rats the vein graft was explanted after being in the arterial system for 4 weeks and was reimplanted as a venous-venous bypass in syngenic Lewis rats. Reimplanted vein grafts (RVGs) were explanted 2 weeks (n = 8) and 8 weeks (n = 8) later. Grafts were analyzed by light and electron microscopy, morphometry, and histochemistry, and were put in organ culture to assess PDGF and bFGF production and mitogenic activity.
We observed MH formation in AVGs and MH regression in RVGs (p < 0.001).PDGF and bFGF production correlated with the degree of MH (p < 0.01). Histochemistry showed PDGF and bFGF in the area of MH in AVG, which disappeared in RVG. Conditioned media from AVG had greater mitogenic activity than RVG or control veins.
MH formation and regression in experimental vein grafts correlate with PDGF and bFGF production.
导致动静脉移植物（AVG）中肌内膜增生（MH）的因素尚不清楚。血小板衍生生长因子（PDGF）和碱性成纤维细胞生长因子（bFGF）是两种对平滑肌细胞有强大促有丝分裂作用的因子，与MH的发生有关。本研究的目的是分析实验性静脉移植物中MH的进展和消退与PDGF和bFGF产生之间的相关性。
在64只近交系Lewis大鼠中，在腹主动脉水平插入一段1厘米长的下腔静脉。下腔静脉段取自同基因大鼠。48只大鼠在手术后3天（n = 8）、7天（n = 8）、4周（n = 24）和12周（n = 8）取出AVG。16只大鼠在静脉移植物在动脉系统中放置4周后取出，并作为静脉-静脉旁路重新植入同基因Lewis大鼠体内。重新植入的静脉移植物（RVG）在2周（n = 8）和8周（n = 8）后取出。通过光镜和电镜、形态计量学和组织化学对移植物进行分析，并置于器官培养中评估PDGF和bFGF的产生及促有丝分裂活性。
我们观察到AVG中形成MH，RVG中MH消退（p < 0.001）。PDGF和bFGF的产生与MH程度相关（p < 0.01）。组织化学显示AVG中MH区域有PDGF和bFGF，而在RVG中消失。AVG的条件培养基比RVG或对照静脉具有更强的促有丝分裂活性。
实验性静脉移植物中MH的形成和消退与PDGF和bFGF的产生相关。
