Randone B, Cucina A, Graziano P, Corvino V, Cavallaro G, Palmieri I, Cavallaro A, Sterpetti A V
Department of Surgery, University of Rome, La Sapienza, Italy.
Eur J Vasc Endovasc Surg. 1998 Nov;16(5):401-7. doi: 10.1016/s1078-5884(98)80007-5.
To determine the role of polyclonal anti-basic Fibroblast Growth Factor (bFGF) antibody in inhibiting the proliferation of smooth muscle cells after experimental polytetrafluorethilene (PTFE) arterial grafting.
In 14 male inbred Lewis rats (weight 250 mg) a 1 cm long segment of PTFE was interposed at the level of abdominal aorta. Animals were randomised to receive polyclonal anti-bFGF antibody (group A: n = seven animals) or aspecific immunoglobulin (group B: n = seven animals). Anti-bFGF antibody or aspecific immunoglublin were given intraperitoneally at the end of operation, and for the first 2 postoperative days. Animals were sacrificed 7 days after surgery, 24 h after intraperitoneal injection of BromodeoxyUridin (BrdU) to label proliferating smooth muscle cells.
One animal in each group died in the immediate postoperative period due to anaesthetic problems. All grafts were patent at the time of sacrifice. BrdU labelling index was statistically higher in the control group B animals at the level of the anastomotic regions (proximal anastomosis: group B 7.9% vs. group A 4.1%. Distal anastomosis: group B 5.1% vs. group A 2.6% p = 0.009) and at the level of PTFE graft (group B 3.8% vs. group A 2.6% p = 0.002), while there was no statistical difference between the control thoracic aorta of the two groups.
bFGF plays a major role in the proliferation of smooth muscle cells at the level of the anastomoses after arterial PTFE grafting. Agents able to block the action of bFGF may be useful in inhibiting the formation of myointimal hyperplasia.
确定多克隆抗碱性成纤维细胞生长因子（bFGF）抗体在实验性聚四氟乙烯（PTFE）动脉移植后抑制平滑肌细胞增殖中的作用。
选取14只雄性近交系Lewis大鼠（体重250mg），在腹主动脉水平植入一段1cm长的PTFE。将动物随机分为两组，分别接受多克隆抗bFGF抗体（A组：n = 7只动物）或非特异性免疫球蛋白（B组：n = 7只动物）。在手术结束时及术后头2天经腹腔给予抗bFGF抗体或非特异性免疫球蛋白。术后7天，在腹腔注射溴脱氧尿苷（BrdU）标记增殖的平滑肌细胞24小时后处死动物。
每组各有1只动物在术后即刻因麻醉问题死亡。处死时所有移植物均通畅。在吻合区域水平（近端吻合口：B组7.9% vs. A组4.1%；远端吻合口：B组5.1% vs. A组2.6%，p = 0.009）以及PTFE移植物水平（B组3.8% vs. A组2.6%，p = 0.002），对照组B组动物的BrdU标记指数在统计学上更高，而两组对照胸主动脉之间无统计学差异。
bFGF在PTFE动脉移植后吻合口处平滑肌细胞的增殖中起主要作用。能够阻断bFGF作用的药物可能有助于抑制肌内膜增生的形成。
