Sheng G, Stern C D
Department of Genetics and Development, Columbia University, 701 West 168th Street #1602, New York, New York 10032, USA.
Mech Dev. 1999 Sep;87(1-2):213-6. doi: 10.1016/s0925-4773(99)00150-1.
We have investigated in detail the expression patterns of two Gata genes, cGata2 and cGata3, during early chick development. In addition to confirming previously described expression of these two genes in developing brain, kidney and blood islands, this study reveals several important novel expression domains during very early stages of development. cGata2 is expressed in the area opaca in pre-primitive streak stages, forming a gradient along the A-P axis (strongest anteriorly). Both genes are expressed strongly in the entire non-neural ectoderm from stage 4+, and neither is expressed in prospective neural plate at any stage. Unlike other previously described non-neural markers, neither gene is expressed in the dorsal neural tube. We also describe dynamic expression of cGata2 and cGata3 during eye, ear and gut development.
我们详细研究了两个Gata基因，即cGata2和cGata3，在鸡早期发育过程中的表达模式。除了证实先前描述的这两个基因在发育中的脑、肾和血岛中的表达外，本研究还揭示了在发育非常早期阶段的几个重要的新表达域。cGata2在原条前阶段的暗区表达，沿前后轴形成梯度（最强烈的在前端）。这两个基因在4+期后的整个非神经外胚层中都强烈表达，并且在任何阶段的预期神经板中都不表达。与其他先前描述的非神经标记不同，这两个基因在背侧神经管中都不表达。我们还描述了cGata2和cGata3在眼、耳和肠道发育过程中的动态表达。
