乳糖操纵子的紧密结合阻遏物：选择系统与体外分析
Tight-binding repressors of the lac operon: selection system and in vitro analysis.
作者信息
Pfahl M
出版信息
J Bacteriol. 1979 Jan;137(1):137-45. doi: 10.1128/jb.137.1.137-145.1979.
Abstract
The isolation and characterization of altered repressors of the lac operon which have an increased affinity for an operator should give useful clues about the molecular basis for the very tight and specific interaction between repressor and operator. A selection system has been devised which allows the isolation of such repressor mutants. This system selects for mutant repressors which can overcome lac operator-constitutive (Oc) mutations. By using in vivo assays, 24 candidates were obtained which, compared with wild type, have an increased trans effect of their repressor on one or several Oc operators. Three of these candidates have been investigated in vitro; the affinity of their repressor for inducer was unchanged, whereas the affinity for wild-type operator was increased 15-, 86-, and 262-fold, respectively.
摘要
对乳糖操纵子中与操纵基因亲和力增加的改变型阻遏物进行分离和特性分析，应能为阻遏物与操纵基因之间非常紧密且特异的相互作用的分子基础提供有用线索。已设计出一种选择系统，可用于分离此类阻遏物突变体。该系统筛选能克服乳糖操纵基因组成型（Oc）突变的突变型阻遏物。通过体内分析，获得了24个候选物，与野生型相比，其阻遏物对一个或几个Oc操纵基因的反式作用增强。已对其中3个候选物进行了体外研究；它们的阻遏物对诱导剂的亲和力未变，而对野生型操纵基因的亲和力分别增加了15倍、86倍和262倍。
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