乳糖阻遏物活性位点的遗传分析。
Genetic analysis of the active sites of lac repressor.
作者信息
Pfahl M, Stockter C, Gronenborn B
出版信息
Genetics. 1974 Apr;76(4):669-79. doi: 10.1093/genetics/76.4.669.
Abstract
Selection methods for the isolation of i(-d) and i(s) mutants are described. Two hundred and forty-seven i(-d) and 98 i(s) mutations have been localized by deletion mapping. All i(-d) mutations map in the region of the i gene, which codes for the aminoterminal part of the lac repressor, whereas i(s) mutations map in the middle of the i gene and at the proximal end of the i(-d) cluster.
摘要
本文描述了分离i(-d)和i(s)突变体的选择方法。通过缺失作图已将247个i(-d)和98个i(s)突变定位。所有i(-d)突变都定位在i基因区域，该基因编码乳糖阻遏物的氨基末端部分，而i(s)突变则定位在i基因的中部和i(-d)簇的近端。
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本文引用的文献
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Isolation of the lac repressor.乳糖阻遏蛋白的分离
Proc Natl Acad Sci U S A. 1966 Dec;56(6):1891-8. doi: 10.1073/pnas.56.6.1891.
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Direction of transcription of a regulatory gene in E. coli.大肠杆菌中调控基因的转录方向。
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Mutants that make more lac repressor.产生更多乳糖阻遏物的突变体。
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The amino-acid sequence of lac repressor.乳糖阻遏蛋白的氨基酸序列。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3576-80. doi: 10.1073/pnas.70.12.3576.
5
Genetic map of the lactose repressor gene (i) of Escherichia coli.大肠杆菌乳糖阻遏蛋白基因（i）的遗传图谱。
Genetics. 1972 Nov;72(3):393-410. doi: 10.1093/genetics/72.3.393.
6
How lac repressor binds to DNA.乳糖阻遏蛋白如何与DNA结合。
Nature. 1972 Jun 9;237(5354):322-7. doi: 10.1038/237322a0.
7
Lac repressor-operator interaction. V. Characterization of super- and pseudo-wild-type repressors.乳糖阻遏物-操纵基因相互作用。V. 超野生型和假野生型阻遏物的特性
J Mol Biol. 1972 Feb 28;64(1):181-99. doi: 10.1016/0022-2836(72)90328-2.
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