Cortés Blanco A, Ferrández Longás A, Mayayo Dehesa E, Labarta Aizpún J I
Hospital Infantil Miguel Servet, Zaragoza.
An Esp Pediatr. 1999 Aug;51(2):159-66.
Our purpose was to estimate reference values for basal serum concentrations of follicle-stimulating hormone (FSH), luteinizing hormone (LH), total testosterone, free testosterone, 17-beta-estradiol (E2) and sex steroid binding globulin (SHBG) in healthy children of Zaragoza.
The reference population consisted of healthy children between 0 and 14 years of age with normal weight and height and living in the metropolitan area of Zaragoza (Spain). It was a transversal study. Basal serum concentrations of FSH, LH and SHBG were measured by immunoradiometric assay. Basal serum concentrations of total testosterone, free testosterone and E2 were analyzed by radioimmunoassay. Reference values and ranges were estimated according to the recommendations of the International Federation of Clinical Chemistry.
Reference values have been classified by age, sex and pubertal stage. Serum concentrations of FSH, LH, total testosterone, free testosterone and E2 increase during the first six months, remain low in infancy and rise during puberty. All of these concentrations showed marked differences according to sex. Serum SHBG levels are influenced by age and during puberty by sex.
Differences in reference values for gonadotrophins, sex steroids and SHBG during infancy, childhood and adolescence makes it necessary for every population to establish their own reference values according to age, sex and pubertal stage.
我们的目的是估算西班牙萨拉戈萨健康儿童基础血清中促卵泡激素（FSH）、促黄体生成素（LH）、总睾酮、游离睾酮、17-β-雌二醇（E2）和性激素结合球蛋白（SHBG）的参考值。
参考人群包括年龄在0至14岁之间、体重和身高正常且居住在萨拉戈萨（西班牙）大都市区的健康儿童。这是一项横断面研究。采用免疫放射分析法测定FSH、LH和SHBG的基础血清浓度。采用放射免疫分析法分析总睾酮、游离睾酮和E2的基础血清浓度。根据国际临床化学联合会的建议估算参考值和范围。
参考值已按年龄、性别和青春期阶段进行分类。FSH、LH、总睾酮、游离睾酮和E2的血清浓度在前六个月升高，在婴儿期保持较低水平，并在青春期升高。所有这些浓度根据性别显示出显著差异。血清SHBG水平受年龄影响，在青春期受性别影响。
婴儿期、儿童期和青春期促性腺激素、性激素和SHBG参考值的差异使得每个群体都有必要根据年龄、性别和青春期阶段建立自己的参考值。
