Balch W E, Wolfe R S
J Bacteriol. 1979 Jan;137(1):256-63. doi: 10.1128/jb.137.1.256-263.1979.
The specificity of the growth requirement of Methanobacterium ruminantium strain M1 for a new coenzyme, 2-mercaptoethanesulfonic acid (HS--CoM), was examined. A variety of derivatives, analogs, and potential biosynthetic precursors of coenzyme M were tested; only a restricted range of thioether, thioester, and thiocarbonate derivatives of the cofactor were found to replace the HS--CoM requirement. Bromoethanesulfonic acid (BrCH2CH2SO3-), a halogenated analog of HS--CoM, potently inhibited the growth response. No coenzyme was detectable in a wide range of nonmethanogenic eucaryotic tissues and procaryotic organisms. However, all methanogens available in pure culture exhibited high levels of coenzyme M which ranged from 0.3 to 16 nmol/mg of dry weight.
对反刍甲烷杆菌菌株M1生长所需的一种新辅酶2-巯基乙烷磺酸（HS-CoM）的特异性进行了研究。测试了辅酶M的多种衍生物、类似物和潜在生物合成前体；发现只有有限范围的该辅因子硫醚、硫酯和硫代碳酸盐衍生物能够替代对HS-CoM的需求。HS-CoM的卤代类似物溴乙烷磺酸（BrCH2CH2SO3-）强烈抑制生长反应。在广泛的非产甲烷真核组织和原核生物中未检测到辅酶。然而，纯培养中可用的所有产甲烷菌都表现出高水平的辅酶M，范围为0.3至16 nmol/mg干重。
