辅酶M衍生物及其对巴氏甲烷八叠球菌细胞提取物由二氧化碳和甲醇生成甲烷的影响。
Coenzyme M derivatives and their effects on methane formation from carbon dioxide and methanol by cell extracts of Methanosarcina barkeri.
作者信息
Hutten T J, De Jong M H, Peeters B P, van der Drift C, Vogels G D
出版信息
J Bacteriol. 1981 Jan;145(1):27-34. doi: 10.1128/jb.145.1.27-34.1981.
Abstract
Extracts of Methanosarcina barkeri reduced methanol and CO2 to CH4 in the presence of H2 and converted methanol stoichiometrically into CH4 and CO2 in the absence of H2. In dialyzed cell-free extracts these reactions were stimulated by 2-mercaptoethanesulfonic acid (coenzyme M) and some derivatives (acetyl and formylcoenzyme M and the oxidized form of coenzyme M), which could be converted to coenzyme M by enzyme systems present in the extracts. Methylcoenzyme M could not be used in these systems.
摘要
巴氏甲烷八叠球菌提取物在有H₂存在的情况下可将甲醇和CO₂还原为CH₄，而在无H₂时可将甲醇化学计量地转化为CH₄和CO₂。在透析后的无细胞提取物中，这些反应受到2-巯基乙烷磺酸（辅酶M）和一些衍生物（乙酰辅酶M、甲酰辅酶M以及辅酶M的氧化形式）的刺激，这些衍生物可被提取物中存在的酶系统转化为辅酶M。甲基辅酶M不能在这些系统中使用。
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