Liaw S J, Teng L J, Ho S W, Luh K T
School of Medical Technology, College of Medicine, National Taiwan University, Taipei, ROC.
J Microbiol Immunol Infect. 1998 Dec;31(4):261-3.
Long-term preservation of 40 isolates of Helicobacter pylori was investigated with five storage media (brucella broth with 17% glycerol, brucella broth with 17% glycerol and 2% fetal calf serum [FCS], brucella broth with 17% glycerol and 10% FCS, brucella broth with 17% glycerol and 2% horse blood, and 10% mucin) at -70 degrees C for 4, 6 and 9 months. In addition to glycerol, FCS or horse blood in the storage media is necessary for survival of H. pylori. Storage of H. pylori isolates at -70 degrees C in 10% mucin is a simple and effective preservation procedure.
研究了用五种储存培养基（含17%甘油的布鲁氏菌肉汤、含17%甘油和2%胎牛血清[FCS]的布鲁氏菌肉汤、含17%甘油和10%FCS的布鲁氏菌肉汤、含17%甘油和2%马血的布鲁氏菌肉汤以及10%粘蛋白）在-70℃下对40株幽门螺杆菌进行4、6和9个月的长期保存。除甘油外，储存培养基中的FCS或马血对幽门螺杆菌的存活是必需的。将幽门螺杆菌菌株在-70℃下保存在10%粘蛋白中是一种简单有效的保存方法。
