Whitmire Jeannette M, Merrell D Scott
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Methods Mol Biol. 2012;921:17-27. doi: 10.1007/978-1-62703-005-2_4.
Half of the world's population is persistently infected with Helicobacter pylori. The chronicity of this infection ultimately elicits clinical manifestations ranging from gastritis and peptic ulcers to adenocarcinoma and MALT lymphoma. Laboratory research following the initial observations of Helicobacter species was greatly hindered by an inability to isolate and culture the bacteria. Thus, the ability to culture bacterial species from this genus is an extremely important step in expanding clinical knowledge and development of therapies. This chapter describes successful techniques for culturing H. pylori on selective horse blood agar media and in Brucella broth liquid media. Additionally, the specific growth requirements of other Helicobacter species are noted.
全球一半人口持续感染幽门螺杆菌。这种感染的慢性病程最终会引发从胃炎、消化性溃疡到腺癌和黏膜相关淋巴组织淋巴瘤等一系列临床表现。在首次观察到幽门螺杆菌后，实验室研究因无法分离和培养该细菌而受到极大阻碍。因此，培养该属细菌的能力是拓展临床知识和开发治疗方法的极其重要的一步。本章介绍了在选择性马血琼脂培养基和布鲁氏菌肉汤液体培养基上培养幽门螺杆菌的成功技术。此外，还提到了其他幽门螺杆菌物种的特定生长需求。
