Bhargava M M, Attia E T, Murrell G A, Dolan M M, Warren R F, Hannafin J A
Laboratory for Soft Tissue Research, Hospital for Special Surgery, New York, New York 10021, USA.
Am J Sports Med. 1999 Sep-Oct;27(5):636-43. doi: 10.1177/03635465990270051601.
We determined the effect of cytokines on the proliferation and migration of cells isolated from the inner-third (white-white), middle-third (red-white), and outer-third (red-red) regions of bovine meniscus. Cells from the outer, or peripheral, region of the meniscus exhibited higher DNA synthesis in the presence of 10% serum compared with cells from the inner or central regions. Recombinant human platelet-derived growth factor-AB, hepatocyte growth factor/scatter factor, and bone morphogenic protein-2 stimulated DNA synthesis of all meniscal cells in a dose-dependent manner, with a two- to threefold maximal stimulation at 10 ng/ml. Cell migration was also stimulated by addition of cytokines. Platelet-derived growth factor and hepatocyte growth factor caused an increase in the migration of cells derived from all three zones, while interleukin-1 selectively stimulated the migration of outer-zone meniscal cells. Epidermal growth factor was much less effective and stimulated the migration of cells in the inner and outer zones by 40% to 50%, while bone morphogenic protein-2 and insulin-like growth factor-1 stimulated the migration of meniscal cells from the middle zone by 40% to 50%. The identification of cytokines that stimulate both the growth and migration of meniscal cells may provide new tools for modulation of meniscal healing.
我们测定了细胞因子对从牛半月板内三分之一（白白）、中三分之一（红白）和外三分之一（红红）区域分离的细胞增殖和迁移的影响。与来自内部或中央区域的细胞相比，半月板外部或周边区域的细胞在存在10%血清的情况下表现出更高的DNA合成。重组人血小板衍生生长因子-AB、肝细胞生长因子/散射因子和骨形态发生蛋白-2以剂量依赖的方式刺激所有半月板细胞的DNA合成，在10 ng/ml时最大刺激为2至3倍。细胞因子的添加也刺激了细胞迁移。血小板衍生生长因子和肝细胞生长因子导致来自所有三个区域的细胞迁移增加，而白细胞介素-1选择性地刺激外部区域半月板细胞的迁移。表皮生长因子的效果要差得多，刺激内部和外部区域细胞的迁移达40%至50%，而骨形态发生蛋白-2和胰岛素样生长因子-1刺激中间区域半月板细胞的迁移达40%至50%。鉴定刺激半月板细胞生长和迁移的细胞因子可能为调节半月板愈合提供新的工具。
