Chen CK, Blaschek HP
Department of Food Science and Human Nutrition, University of Illinois, Urbana 61801, USA.
Appl Microbiol Biotechnol. 1999 Aug;52(2):170-3. doi: 10.1007/s002530051504.
Addition of sodium acetate to chemically defined MP2 medium was found to increase and stabilize solvent production by Clostridium beijerinckii BA101, a solvent-hyperproducing mutant derived from C. beijerinckii NCIMB 8052. C. beijerinckii BA101 demonstrated a greater increase in solvent production than C. beijerinckii NCIMB 8052 when sodium acetate was added to MP2 medium. In 1-l batch fermentations, C. beijerinckii BA101 produced 32.6 g/l total solvents, with butanol at 20.9 g/l, when grown in MP2 medium containing 60 mM sodium acetate and 8% glucose. To our knowledge, these values represent the highest solvent and butanol concentrations produced by a solventogenic Clostridium strain when grown in batch culture.
研究发现，向化学成分明确的MP2培养基中添加醋酸钠可增加并稳定拜氏梭菌BA101（一种源自拜氏梭菌NCIMB 8052的溶剂高产突变体）的溶剂产量。当向MP2培养基中添加醋酸钠时，拜氏梭菌BA101的溶剂产量增幅大于拜氏梭菌NCIMB 8052。在1升分批发酵中，拜氏梭菌BA101在含有60 mM醋酸钠和8%葡萄糖的MP2培养基中生长时，可产生32.6克/升的总溶剂，其中丁醇含量为20.9克/升。据我们所知，这些数值代表了产溶剂梭菌菌株在分批培养时产生的最高溶剂和丁醇浓度。
