大豆糖蜜作为拜氏梭菌BA101生产丁醇的发酵底物。

Soy molasses as fermentation substrate for production of butanol using Clostridium beijerinckii BA101.

作者信息

Qureshi N, Lolas A, Blaschek H P

机构信息

Biotechnology and Bioengineering Group, Department of Food Science and Human Nutrition, University of Illinois, 1207 West Gregory Drive, Urbana, IL 61801, USA.

出版信息

J Ind Microbiol Biotechnol. 2001 May;26(5):290-5. doi: 10.1038/sj.jim.7000131.

DOI:10.1038/sj.jim.7000131

PMID:11494105

Abstract

Spray-dried soy molasses (SDSM) contains the sugars dextrose, sucrose, fructose, pinitol, raffinose, verbascose, melibiose, and stachyose. Of the 746 g kg(-1) total sugars in SDSM, 434 g kg(-1) is fermentable using Clostridium beijerinckii BA101. SDSM was used to produce acetone, butanol, and ethanol (ABE) by C. beijerinckii BA101 in batch cultures. Using 80 g l(-1) SDSM, 10.7 g l(-1) ABE was produced in P2 medium. Higher concentrations of SDSM resulted in poor solvent production due to the presence of excessive salt and inhibitory components. C. beijerinckii BA101 in SDSM at 80 g l(-1) concentration produced 22.8 g l(-1) ABE when supplemented with 25.3 g l(-1) glucose. SDSM contains 57.4 g kg(-1) mineral ash and 2% tri-calcium phosphate. Tri-calcium phosphate up to 43.1 g l(-1) was not inhibitory and at a tri-calcium phosphate concentration of 28.8 g l(-1), the culture produced more solvents (30.1 g l(-1)) than the control experiment (23.8 g l(-1)). In contrast, sodium chloride was a strong inhibitor of C. beijerinckii BA101 cell growth. At a concentration of 10 g l(-1) sodium chloride, a maximum cell concentration of 0.6 g l(-1) was achieved compared to 1.7 g l(-1) in the control experiment. The effects of two salts on specific growth rate constant (mu) and specific rate of ABE production (nu) for C. beijerinckii BA101 were examined.

摘要

喷雾干燥大豆糖蜜（SDSM）含有葡萄糖、蔗糖、果糖、松醇、棉子糖、毛蕊花糖、蜜二糖和水苏糖等糖类。在SDSM的746 g kg⁻¹总糖中，434 g kg⁻¹可被拜氏梭菌BA101发酵利用。SDSM被用于在分批培养中由拜氏梭菌BA101生产丙酮、丁醇和乙醇（ABE）。在P2培养基中使用80 g l⁻¹的SDSM，可产生10.7 g l⁻¹的ABE。由于存在过量盐分和抑制性成分，较高浓度的SDSM导致溶剂产量不佳。当添加25.3 g l⁻¹葡萄糖时，浓度为80 g l⁻¹的SDSM中的拜氏梭菌BA101产生了22.8 g l⁻¹的ABE。SDSM含有57.4 g kg⁻¹的矿物灰分和2%的磷酸三钙。高达43.1 g l⁻¹的磷酸三钙没有抑制作用，在磷酸三钙浓度为28.8 g l⁻¹时，培养物产生的溶剂（30.1 g l⁻¹）比对照实验（23.8 g l⁻¹）更多。相比之下，氯化钠是拜氏梭菌BA101细胞生长的强抑制剂。在氯化钠浓度为10 g l⁻¹时，最大细胞浓度达到0.6 g l⁻¹，而对照实验中为1.7 g l⁻¹。研究了两种盐对拜氏梭菌BA101的比生长速率常数（μ）和ABE比生产速率（ν）的影响。