Lewis R A, Curnock S P, Dyke K G
Department of Biochemistry, University of Oxford, UK.
FEMS Microbiol Lett. 1999 Sep 15;178(2):271-5. doi: 10.1111/j.1574-6968.1999.tb08687.x.
The proteolytic cleavage of BlaI was shown to correlate with beta-lactamase synthesis in Staphylococcus aureus. BlaI was found to be autoregulatory when expressed from the blaZ promoter. Insertion of a 10-bp linker into the SnaBI site of blaRI resulted in constitutive synthesis of beta-lactamase.
已证明BlaI的蛋白水解切割与金黄色葡萄球菌中β-内酰胺酶的合成相关。当从blaZ启动子表达时，发现BlaI具有自我调节作用。在blaRI的SnaBI位点插入一个10个碱基对的接头导致β-内酰胺酶的组成型合成。
