Clarke Simon R, Dyke Keith G H
Microbiology Unit, Department of Biochemistry, South Parks Road, Oxford OX1 3QU, UK1.
Microbiology (Reading). 2001 Apr;147(Pt 4):803-810. doi: 10.1099/00221287-147-4-803.
The precise start points for transcription of the blaZ and of the blaRI/blaI genes of the Staphylococcus aureus beta-lactamase operon have been determined by primer extension analysis. Consequently the overlapping promoter sequences were deduced. Northern blots showed that the synthesis of the 2100 nt mRNA from blaRI is inducible and that a blaI probe hybridized to the same mRNA as the blaRI probe. The gene cat, encoding chloramphenicol acetyltransferase, was fused separately to the blaZ and blaRI/blaI promoters, and used to compare their strengths. The promoter for blaZ is about six times stronger than that for blaRI/blaI and the synthesis of chloramphenicol acetyltransferase from both promoters is inducible, supporting the results from the Northern blots.
通过引物延伸分析确定了金黄色葡萄球菌β-内酰胺酶操纵子中blaZ基因以及blaRI/blaI基因转录的精确起始点。由此推导得出重叠的启动子序列。Northern印迹显示，来自blaRI的2100 nt mRNA的合成是可诱导的，并且bla I探针与和blaRI探针相同的mRNA杂交。编码氯霉素乙酰转移酶的cat基因分别与blaZ和blaRI/blaI启动子融合，并用于比较它们的强度。blaZ的启动子比blaRI/blaI的启动子强约六倍，并且来自这两个启动子的氯霉素乙酰转移酶的合成都是可诱导的，这支持了Northern印迹的结果。
