PM2的全基因组序列，首个被分离出来的含脂质细菌病毒。

The complete genome sequence of PM2, the first lipid-containing bacterial virus To Be isolated.

作者信息

Männistö R H, Kivelä H M, Paulin L, Bamford D H, Bamford J K

机构信息

Department of Biosciences and Institute of Biotechnology, Viikki Biocenter, Helsinki, FIN-00014, Finland.

出版信息

Virology. 1999 Sep 30;262(2):355-63. doi: 10.1006/viro.1999.9837.

DOI:10.1006/viro.1999.9837

PMID:10502514

Abstract

Bacteriophage PM2 was isolated from the Pacific Ocean off the coast of Chile in the late 1960s. It was a new virus type, later classified as Corticoviridae, and also the first bacterial virus for which it was demonstrated that lipids are part of the virion structure. Here we report the determination and analysis of the 10, 079-bp circular dsDNA genome sequence. Noteworthy discoveries are the replication initiation system, which is related to the rolling circle mechanism described for phages such as φX174 and P2, and a 1.2-kb sequence that is similar to the maintenance region of a plasmid found in a marine Pseudoalteromonas sp. strain A28.

摘要

噬菌体PM2于20世纪60年代末从智利海岸附近的太平洋中分离出来。它是一种新型病毒，后来被归类为复层噬菌体科，也是首个被证明脂质是病毒粒子结构一部分的细菌病毒。在此，我们报告了对其10,079碱基对环状双链DNA基因组序列的测定与分析。值得注意的发现包括与诸如φX174和P2等噬菌体所描述的滚环机制相关的复制起始系统，以及一段与在海洋假交替单胞菌属菌株A28中发现的一种质粒的维持区域相似的1.2千碱基对序列。