Garner R C, Lightfoot T J, Cupid B C, Russell D, Coxhead J M, Kutschera W, Priller A, Rom W, Steier P, Alexander D J, Leveson S H, Dingley K H, Mauthe R J, Turteltaub K W
JBUEC, Department of Biology, University of York, UK.
Cancer Lett. 1999 Sep 1;143(2):161-5. doi: 10.1016/s0304-3835(99)00118-4.
MeIQx and PhIP are putative carcinogenic heterocyclic amines formed during the cooking of meat and fish. Using accelerator mass spectrometry, we have investigated the metabolism and macromolecule binding of 14C-labelled MeIQx and PhIP in human cancer patients compared to the rat. Following oral administration of MeIQx and PhIP, more DNA adducts were formed in human colon tissue compared with rats. Differences were also observed between rats and humans in the metabolite profile and urine excretion for these compounds. These results suggest humans metabolise heterocyclic amines differently to laboratory rodents and question their use as models of human risk.
MeIQx和PhIP是在肉类和鱼类烹饪过程中形成的潜在致癌杂环胺。我们使用加速器质谱法，研究了与大鼠相比，14C标记的MeIQx和PhIP在人类癌症患者体内的代谢及与大分子的结合情况。口服MeIQx和PhIP后，与大鼠相比，人类结肠组织中形成的DNA加合物更多。在这些化合物的代谢产物谱和尿液排泄方面，大鼠和人类之间也存在差异。这些结果表明，人类对杂环胺的代谢方式与实验啮齿动物不同，并对将它们用作人类风险模型提出了质疑。
