Boctor F N, Nash T E, Cheever A W
J Immunol. 1979 Jan;122(1):39-43.
A polysaccharide antigen was isolated from Schistosoma mansoni egg homogenates by the phenol procedure. The crude preparation (CPEA) contained at least two antigens. The more purified antigen (PEA) was isolated by sequential enzymatic treatment of CPEA with DNase, RNase, Pronase, and alpha-amylase. PEA was resistant to boiling, freezing and thawing, mild acid and alkali, and chloroform, but was destroyed with periodate. It gave a positive reaction with anthrone reagent. PEA was eluted in the wash fraction from a DEAE cellulose collumn and in the void volume of a Sephacryl 200 column. After immunoelectrophoresis and polyacrylamide electrophoresis there was little or no migration. Amino acid analysis failed to reveal ninhydrin-positive material in the a hydrolyzate of PEA. These resluts suggested that PEA is a neutral polysaccharide with a m.w. of more than 200,000 and contains no amino acids or hexosamine. Antibodies against PEA were detected in sera obtained from mice infected with S. mansoni. PEA is different from previously described antigens derived from schistosome eggs.
通过苯酚法从曼氏血吸虫卵匀浆中分离出一种多糖抗原。粗制品（CPEA）含有至少两种抗原。通过用脱氧核糖核酸酶、核糖核酸酶、链霉蛋白酶和α-淀粉酶对CPEA进行顺序酶处理，分离出了更纯的抗原（PEA）。PEA对煮沸、冷冻和解冻、弱酸和弱碱以及氯仿具有抗性，但会被高碘酸盐破坏。它与蒽酮试剂呈阳性反应。PEA从DEAE纤维素柱的洗脱部分以及Sephacryl 200柱的空体积中洗脱出来。免疫电泳和聚丙烯酰胺电泳后几乎没有迁移。氨基酸分析未能在PEA的水解产物中检测到茚三酮阳性物质。这些结果表明，PEA是一种分子量超过200,000的中性多糖，不含氨基酸或己糖胺。在感染曼氏血吸虫的小鼠血清中检测到了针对PEA的抗体。PEA与先前描述的源自血吸虫卵的抗原不同。
