Brown A P, Remold H G, Warren K S, David J R
J Immunol. 1977 Oct;119(4):1275-8.
Schistosoma mansoni egg antigens that elicit delayed hypersensitivity in appropriately sensitized guinea pigs were partially characterized by using ion exchange chromatography and preparative electrophoresis. At least three skin-reactive antigens were found, one of which was purified to homogeneity, as analyzed by polyacrylamide gel electrophoresis (PAGE). This antigen was not adsorbed to CM cellulose, migrated cathodal to guinea pig albumin on electrophoresis, and was adsorbed to DEAE cellulose. A second pool of antigenic activity was obtained by adsorption to CM cellulose and subsequent elution. DEAE cellulose chromatography and preparative electrophoresis of this pool indicated the presence of more than one antigen.
利用离子交换色谱法和制备电泳法对曼氏血吸虫卵抗原进行了部分特性分析，这些抗原能在适当致敏的豚鼠中引发迟发型超敏反应。发现了至少三种皮肤反应性抗原，其中一种经聚丙烯酰胺凝胶电泳（PAGE）分析已纯化至同质。该抗原不吸附于CM纤维素，在电泳时向阴极迁移至豚鼠白蛋白位置，并吸附于DEAE纤维素。通过吸附于CM纤维素并随后洗脱获得了第二组抗原活性。对该组抗原进行DEAE纤维素色谱分析和制备电泳表明存在不止一种抗原。
