Harrison D J, Carter C E, Colley D G
J Immunol. 1979 Jun;122(6):2210-7.
Schistosoma mansoni egg antigens were purified from a heterogeneous mixture of soluble egg antigens (crude SEA) with an immunoaffinity column that consisted of the specific anti-SEA antibodies contained in 16-week S. mansoni-infected mouse serum bound to Sepharose 4B. On sodium dodecyl sulfate (SDS) gel electrophoresis, the purified antigen fraction yielded at least eight bands staining with Coomassie blue and at least five bands staining with Coomaisse blue and at least five bands reacting with periodic acid-Schiff (PAS). All of the proteins in the antigenic fraction appear to contain carbohydrate residues. Upon immunoelectrophoresis the antigen yielded four precipitin arcs. The antigenic fraction isolated by means of the immunoaffinity column was then compared to various fractions obtained from concanavalin A (Con A) chromatography of SEA. The results of Ouchterlony immunodiffusion and immunoelectrophoresis indicate that the antigenic fraction isolated by immunoaffinity purification of SEA contains the major antigens found in the fractions obtained from Con A chromatography of SEA. The results of SDS gel electrophoresis indicate that the major PAS-reacting bands of the antigenic fraction isolated by immunoaffinity purification are found in the 3rd peak (bound fraction) resulting from Con A chromatography of SEA, whereas the major Coomaisse blue-staining band in the isolated antigenic fraction is found in the 2nd peak (unbound fraction) from Con A chromatography of SEA.
曼氏血吸虫卵抗原是从可溶性卵抗原（粗制SEA）的异质混合物中，通过免疫亲和柱进行纯化的。该免疫亲和柱由结合在琼脂糖4B上的、来自感染曼氏血吸虫16周的小鼠血清中所含的特异性抗SEA抗体组成。在十二烷基硫酸钠（SDS）凝胶电泳中，纯化的抗原组分产生至少八条考马斯亮蓝染色带以及至少五条过碘酸-希夫（PAS）反应带。抗原组分中的所有蛋白质似乎都含有碳水化合物残基。在免疫电泳中，该抗原产生四条沉淀弧。然后将通过免疫亲和柱分离得到的抗原组分与从SEA的伴刀豆球蛋白A（Con A）层析获得的各种组分进行比较。双向免疫扩散和免疫电泳结果表明，通过SEA免疫亲和纯化分离得到的抗原组分包含从SEA的Con A层析获得的组分中所发现的主要抗原。SDS凝胶电泳结果表明，通过免疫亲和纯化分离得到的抗原组分的主要PAS反应带位于SEA的Con A层析产生的第3个峰（结合组分）中，而分离的抗原组分中的主要考马斯亮蓝染色带位于SEA的Con A层析的第2个峰（未结合组分）中。
