Kiho T, Ookubo K, Usui S, Ukai S, Hirano K
Department of Pharmaceutics, Gifu Pharmaceutical University, Japan.
Biol Pharm Bull. 1999 Sep;22(9):966-70. doi: 10.1248/bpb.22.966.
A polysaccharide (CS-F10) purified from a hot water extract of the cultured mycelium of Cordyceps sinensis was composed of galactose, glucose and mannose in a molar ratio of 43:33:24; its molecular weight was estimated to be about 15000. The results of chemical and spectroscopic investigations suggest that CS-F10 has a comb-type structure, and has alpha-D-glucopyranosyl residues on the terminal of the side-chains and characteristic sugar residues of C. sinensis i.e., 1,5-linked beta-D-galactofuranosyl residues. CS-F10 significantly lowered the plasma glucose level in normal, streptozotocin (STZ)-induced diabetic and epinephrine-induced hyperglycemic mice after intraperitoneal administration (50 mg/kg). Administration of CS-F10 to STZ-induced diabetic mice significantly increased the activity of hepatic glucokinase. A significant reduction in the hepatic glucose output was observed following the infusion of CS-F10 using the perfused rat liver. CS-F10 also significantly decreased protein content of facilitative glucose transporter isoform 2 (GLUT2) from rat liver following i.p. administration. These effects presumably contribute to the hypoglycemic activity.
从中华虫草培养菌丝体的热水提取物中纯化得到的一种多糖（CS-F10），其由半乳糖、葡萄糖和甘露糖以摩尔比43:33:24组成；其分子量估计约为15000。化学和光谱研究结果表明，CS-F10具有梳状结构，在侧链末端具有α-D-吡喃葡萄糖基残基以及中华虫草的特征性糖残基，即1,5-连接的β-D-呋喃半乳糖基残基。腹腔注射（50mg/kg）后，CS-F10能显著降低正常小鼠、链脲佐菌素（STZ）诱导的糖尿病小鼠和肾上腺素诱导的高血糖小鼠的血糖水平。给STZ诱导的糖尿病小鼠注射CS-F10能显著提高肝葡萄糖激酶的活性。使用灌注大鼠肝脏灌注CS-F10后，观察到肝葡萄糖输出量显著降低。腹腔注射后，CS-F10还能显著降低大鼠肝脏中葡萄糖转运蛋白异构体2（GLUT2）的蛋白质含量。这些作用可能有助于其降血糖活性。
