Ito M, Kondo Y, Nakatani A, Naruse A
Department of Pharmacology, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
Biol Pharm Bull. 1999 Sep;22(9):988-9. doi: 10.1248/bpb.22.988.
This study was designed to clarify the relationship between streptozotocin (STZ) dosage (100, 150 and 200 mg/kg i.p.) and the resulting diabetogenic response in mice (8-week-old male ICR). In this experiment, we found that a single i.p. injection of 100 mg/kg STZ is able to induce progressive diabetes mellitus, in which non-fasting serum glucose levels begin to rise from 3 weeks and continue to rise throughout the experimental period until 9 weeks. The non-fasting serum insulin levels of 100 mg/kg STZ-treated mice were normal during the experimental period. In addition, the population of insulin-immunoreactive cells (beta cells) in the islets of pancreata was slightly less than in normal mice at 9 weeks. In 200 mg/kg STZ-treated mice, on the other hand, the insulin levels were below measurable values and insulin-immunoreactive cells were not observed. It is concluded from these results that the progressive diabetic mouse model induced by a single i.p. injection of 100 mg/kg STZ, unlike 200 mg/kg STZ-induced diabetes which is insulin-dependent, is non-insulin-dependent.
本研究旨在阐明链脲佐菌素（STZ）剂量（100、150和200mg/kg腹腔注射）与8周龄雄性ICR小鼠由此产生的致糖尿病反应之间的关系。在本实验中，我们发现单次腹腔注射100mg/kg STZ能够诱发进行性糖尿病，其中非空腹血清葡萄糖水平从第3周开始升高，并在整个实验期持续升高直至第9周。100mg/kg STZ处理的小鼠在实验期内非空腹血清胰岛素水平正常。此外，在第9周时，胰腺胰岛中胰岛素免疫反应性细胞（β细胞）的数量略少于正常小鼠。另一方面，在200mg/kg STZ处理的小鼠中，胰岛素水平低于可测量值，且未观察到胰岛素免疫反应性细胞。从这些结果得出结论，单次腹腔注射100mg/kg STZ诱导的进行性糖尿病小鼠模型与200mg/kg STZ诱导胰岛素依赖型糖尿病不同，是非胰岛素依赖型的。
