Ito M, Kondo Y, Nakatani A, Hayashi K, Naruse A
Laboratory of Analytical Pharmacology, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tenpaku-ku, 468-8503, Nagoya, Japan
Environ Toxicol Pharmacol. 2001 Jan 1;9(3):71-78. doi: 10.1016/s1382-6689(00)00064-8.
Our previous study indicated that a single i.p. injection of 100 mg/kg streptozotocin (STZ) is able to induce slowly progressive diabetes mellitus in adult ICR mice. In the present study, the non-fasting serum insulin levels of the mice administered 100 mg/kg STZ were normal throughout the 24-week-observation after STZ injection. In the STZ-administered mice, the area of islets and the number of insulin-immunoreactive cells (beta-cells) were normal at 1 week and then continued to decrease gradually as the time went on. In contrast, there was a relative increase in the number of glucagon-immunoreactive cells (alpha-cells) in these mice. In addition, in the STZ-administered mice, the degree of glucose tolerance continued to reduce from 2 weeks till 12 weeks when the experiment terminated. The rise in serum insulin levels stimulated by glucose in the STZ-administered mice began to subside from about 2 weeks and had completely ceased by 12 weeks. These results indicate that 100 mg/kg STZ-induced diabetic mouse model is non-insulin-dependent diabetes, which is characterized by impaired insulin response to glucose stimulation.
我们之前的研究表明，腹腔注射一次100 mg/kg链脲佐菌素（STZ）能够在成年ICR小鼠中诱导出缓慢进展的糖尿病。在本研究中，注射STZ后24周的观察期内，给予100 mg/kg STZ的小鼠非空腹血清胰岛素水平均正常。在给予STZ的小鼠中，胰岛面积和胰岛素免疫反应性细胞（β细胞）数量在1周时正常，随后随着时间的推移逐渐持续减少。相比之下，这些小鼠中胰高血糖素免疫反应性细胞（α细胞）数量相对增加。此外，在给予STZ的小鼠中，葡萄糖耐量从2周持续降低至实验结束时的12周。给予STZ的小鼠中由葡萄糖刺激引起的血清胰岛素水平升高从约2周开始消退，到12周时完全停止。这些结果表明，100 mg/kg STZ诱导的糖尿病小鼠模型是非胰岛素依赖型糖尿病，其特征是胰岛素对葡萄糖刺激的反应受损。
