Yamagami T, ten Boekel E, Schaniel C, Andersson J, Rolink A, Melchers F
Basel Institute for Immunology, Switzerland.
Immunity. 1999 Sep;11(3):309-16. doi: 10.1016/s1074-7613(00)80106-5.
Single cell PCR assays have been further developed that detect over 80% of all VkappaJkappa, VkappaRS, and VlambdaJlambda rearrangements at efficiencies between 70% and 90%. These IgL chain gene rearrangement assays were used with small pre-BII cells that develop in comparably high numbers in the bone marrow of wild-type, Ckappa-deficient, and JCkappa-deficient homozygous and heterozygous mice. In all of these mice, only 15%-25% of all small pre-BII cells carry VlambdaJlambda rearrangements. These results confirm that lambdaL chain gene rearrangements occur independently of kappaL chain gene rearrangement and expression. They also show that a large part of the small pre-BII cells that express the rearrangement machinery can develop without IgL chain gene rearrangements.
单细胞PCR检测方法已得到进一步发展，其能够以70%至90%的效率检测出所有VκJκ、VκRS和VλJλ重排中的80%以上。这些IgL链基因重排检测方法用于小前BII细胞，这些细胞在野生型、Cκ缺陷型、Jκ缺陷型纯合和杂合小鼠的骨髓中大量发育。在所有这些小鼠中，所有小前BII细胞中只有15%-25%携带VλJλ重排。这些结果证实λL链基因重排独立于κL链基因重排和表达而发生。它们还表明，表达重排机制的大部分小前BII细胞可以在没有IgL链基因重排的情况下发育。
