Yamagami T, ten Boekel E, Andersson J, Rolink A, Melchers F
Basel Institute for Immunology, Switzerland.
Immunity. 1999 Sep;11(3):317-27. doi: 10.1016/s1074-7613(00)80107-7.
PCR analyses of the kappaL chain locus in single B-lineage cells of wild-type, Ckappa-, or JCkappa-deficient homozygous or heterozygous mice often detect multiple in- and out-of-frame rearrangements at the kappaL and lambdaL loci. They are most frequent in small pre-BII cells and equally so in wild-type and kappaL chain-deficient cells. Hence, kappaL chain production appears not to inhibit secondary rearrangements. Around 20% of all small preBII cells express IgL chains in their cytoplasm. Cells with a first productive rearrangement on one allele are favored to enter the immature B cell compartment. Thus, allelic exclusion might be secured by control of accessibility of IgL chain loci for rearrangement and by rapid selection of cells with a fitting over those with a nonfitting IgL chain.
对野生型、Ckappa基因缺失型或JCkappa基因缺失型纯合或杂合小鼠的单个B细胞系细胞中的kappaL链基因座进行PCR分析时，经常会在kappaL和lambdaL基因座检测到多个框内和框外重排。这些重排在小前BII细胞中最为常见，在野生型和kappaL链缺陷型细胞中出现的频率相同。因此，kappaL链的产生似乎不会抑制二次重排。在所有小前BII细胞中，约20%的细胞在其细胞质中表达IgL链。在一个等位基因上发生首次有效重排的细胞更倾向于进入未成熟B细胞区室。因此，等位基因排斥可能通过控制IgL链基因座的重排可及性以及通过快速选择具有合适IgL链的细胞而非不合适IgL链的细胞来实现。
