白色念珠菌和解脂耶氏酵母这两种双态酵母的SLA2序列分析。
Sequence analysis of SLA2 of the dimorphic yeasts Candida albicans and Yarrowia lipolytica.
作者信息
Melms A S, Gausmann U, Swoboda R K, Dominguez A, Kurischko C
机构信息
Hans-Knöll-Institut für Naturstoff-Forschung e.V., Beutenbergstrasse 11, D-07745 Jena, Germany.
出版信息
Yeast. 1999 Oct;15(14):1519-28. doi: 10.1002/(SICI)1097-0061(199910)15:14<1519::AID-YEA475>3.0.CO;2-2.
We report the complete nucleotide sequence of SLA2 of the dimorphic yeasts Candida albicans and Yarrowia lipolytica. In Saccharomyces cerevisiae, SLA2 codes for an actin binding protein. The deduced amino acid (aa) sequences of C. albicans CaSla2p and Y. lipolytica YlSla2p consist of 1063 and 1054 aa, respectively. The alignment of the deduced proteins of Saccharomyces cerevisiae, Y. lipolytica and C. albicans shows regions of identity in the N-terminal part of the proteins, which are essential for growth at 37 degrees C, endocytosis and actin organization in S. cerevisiae. The Sla2p proteins have also several conserved regions in the C-terminal moiety, the I/LWEQ boxes, displaying homology to the talin protein of mouse, Dictyostelium discoideum, Caenorhabditis elegans and to human huntingtin interacting protein (Hip 1p). The sequence data of C. albicans SLA2 are registered in the EMBL database (AJ009556), and for the Y. lipolytica gene in GenBank (U65409).
我们报道了二态性酵母白色念珠菌和解脂耶氏酵母的SLA2的完整核苷酸序列。在酿酒酵母中，SLA2编码一种肌动蛋白结合蛋白。白色念珠菌CaSla2p和解脂耶氏酵母YlSla2p推导的氨基酸（aa）序列分别由1063个和1054个氨基酸组成。酿酒酵母、解脂耶氏酵母和白色念珠菌推导蛋白的比对显示，这些蛋白在N端部分存在相同区域，这对于酿酒酵母在37℃下生长、胞吞作用和肌动蛋白组织至关重要。Sla2p蛋白在C端部分也有几个保守区域，即I/LWEQ框，与小鼠、盘基网柄菌、秀丽隐杆线虫的踝蛋白以及人类亨廷顿相互作用蛋白（Hip 1p）具有同源性。白色念珠菌SLA2的序列数据已登记在EMBL数据库（AJ009556）中，解脂耶氏酵母基因的序列数据已登记在GenBank（U65409）中。
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